Follow up analysis Patients n = 155 blood count at initial examination n = 89 Significant GPI-AP deficient clone and blood count at initial examination n = 36 Neutrophiles < 0,5 G/l n = 8 Thrombocytes < 100 G/l n = 20 No GPI-AP deficient clone and blood count at initial examination n = 53 Neutrophiles < 0,5 G/l n = 24 Thrombocytes < 100 G/l n = 35 blood count at last examination n = 120 Significant GPI-AP deficient clone and blood count at last examination n = 56 Neutrophiles < 0,5 G/l n = 7 Thrombocytes < 100 G/l n = 22 No GPI-AP deficient clone and blood count at last examination n = 64 Neutrophiles < 0,5 G/l n = 14 Thrombocytes < 100 G/l n = 29 Data supplements 1: Flow sheet additional cytopenias in follow up pts

Data supplements 2: a case report of false positive interpretation of significant GPI-deficient populations on leucocytes: patient was diagnosed in an external labaratory to have a significant PNH and eculizumab therapy was started. After the patient developed an ischemic stroke a blood sample was taken and sent to us for confirmation of the diagnosis. Due to the large GPI- deficient populations on the monocytes in combination with the almost normal reticulocytes we suspected immature myeloid cells. Consecutively a bone marrow assessment confirmed an AML M7 with a blast excess and a chemotherapy was started. MonocytesReticulocytes