Proteins: Structure, Chemical Identification & Polypeptide Synthesis 1.Using Biuret to Identify Presence of a Protein 2.Learning the Chemical Structure of an Amino Acid 3.Using a Dehydration Synthesis to Make a Polypeptide Chaine

Testing for Organic Compounds Part A: Protein Testing 1. Involves an indicator, Biruet Solution 2. Biuret reacts with the amino acids in the protein 3. Solution color goes from blue to violet 4. Color change indicates a chemical reaction between the biuret and the bonds between the amino acids of a protein

Another Look at Biuret Test Chemistry of the Biuret Test Negative Control Postive Control Experiment

Proteins Are organic polymers composed of monomers called amino acids. Strands of amino acids are called polypeptide chains. Where are the peptide bonds? Proteins are many chained structures.

Amino Acids Polypeptide Chain Protein with two Polypeptide chains Where is the complete protein?

Parts of an Amino Acid

Here is another diagram of an amino acid….

Lab Safety & Cleanup Test tubes are glass. Biruet Solution is a base. Wash hands with water. Goggles on at all times Use special glassware soap and water with test tube brush to wash all test tube Return all materials to appropriate location Complete data table. On back of lab, construct (5) observation statements and then write a conclusion.

Polypeptide Synthesis Involves two amino acids. Involves a dehydration synthesis. Involves a chemical reaction that occurs between two specific areas of the amino acid. Requires an –OH group and an –H from another –OH group

Functions of Proteins