System Engineering M2D3 11.03.09. Three part lab today Part 1: finish Western Part 2:  -gal assay for your mutants Part 3: sequence analysis.

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Presentation transcript:

System Engineering M2D

Three part lab today Part 1: finish Western Part 2:  -gal assay for your mutants Part 3: sequence analysis

Part 1: finish Western Why are we performing a Western blot?

Part 2:  -gal assay Why are we testing both light and dark grown cells? Miller units Should we test + and – HA tag? How many replicates should we test?

Part 3: Sequence analysis 1.Retrieve the sequences 2.find the reverse complement 3.BLAST your sequence (= query) vs pCph8 (= subject)

Part 3: Sequence analysis