Enzymes A review of how they work

Generate a definition of Enzymes Proteins that promote specific rxns in cells by lowering activation energy required to start a rxn.

How do enzymes work? Lower activation energy increase speed of metabolic rates Break down….build up???

Anatomy of Enzyme Action

Induced fit model Enzymes are specific ench/lab2/induced.html ench/lab2/induced.html hill.com/sites/ /student_view0/chapter2/a nimation__how_enzymes_work.html hill.com/sites/ /student_view0/chapter2/a nimation__how_enzymes_work.html

What happens after an enzyme does its job? Required in small amounts because as they work, not consumed.

Factors that affect how enzymes work? Temperature pH Amount of substrate available

Brainstorm of Enzymes Saliva Stomach Nervous system: AcHase Milk products Papaya fruit Unprocessed fruits Kiwi: actiniclin Soybeans (lipoxygenase) Fireflies (luciferase) Pineapples (bromelain) Potatoes Egg whites Turnips (peroxidase) Tears Yeast (sucrase) Lactase Onions detergents

How do you know “enzymes” are working?

Challenge Design an experiment that will test the effectiveness of an enzyme on the protein gelatin. Stipulations – Experiment will be performed in class period – conduction is totally designed by experimenters – Enzyme will be proteases (source: detergent) – Data collection must be qualitative and quantitative Review scoring guide for how you will be graded

materials Test tubes Petri dishes gelatin Water Detergent (u supply) Ruler Straws Sharpie/wax pens

Exit slip List of materials Independent variable Dependent variable Constants Control Experimental (treatment group) What are you measuring

science/biology/taters/enzyme_mc.htm