Introduction microscopy Introduction microscopy most cells are mm unaided eye sees.1mm Hooke built microscope (30X)

Purpose of Microscope magnification enlarged image by a factor resolving power ability to separate two adjacent points in a specimen contrast how well details stand out against the background parfocal remain in focus when switching objectives

Rules and Care of the Microscope, abridged carry microscope correctly clean lenses with lens paper and slides with Kimwipes set smallest objective in place (4X) and bring stage close of objectives use oil with oil immersion lens (100X) only clean all lenses when finished have instructor check scope before storing

The Compound Microscope Metric measurement of a field of view ocular = 10X mag of lens total mag. (ocular x obj.) 4X 40X 10X 100X 40X 400X 100X 1000X

6 Exercises Ex. 7.1 fill in table Ex. 7.2 depth of focus: colored threads,determine order Ex. 7.3 image orientation letter “e” ; draw at 40x and 100x Ex. 7.4 dissecting microscope Observe bacterial culture dish

Other Microscopes dissecting,greater working distance,reflected and transmitted light light microscope phase contrast microscope transmission electron microscope, TEM scanning electron microscope, SEM

Cell Theory all organisms are made of cells the cell is the smallest living thing all cells arise from pre-existing cells

Eukaryotic Cells Plant : Elodea cells w/ water draw and 400X total mag. Label nucleus,plasma membrane cytoplasm w/ cytosol,cell wall of cellulose Chloroplast,vacuole

Eukaryotic Cells Plant : Onion bulb cellsw/ iodine draw and 400X total mag. Label nucleus w/ DNA,plasma membrane cytoplasm w/ cytosol,cell wall of cellulose Nucleolus,vacuole

Eukaryotic Cells Animal : human cheek cells w/ methylene blue stain draw and 400X total mag. nucleus w/ DNA, plasma membrane,cytoplasm w/ cytosol

Prokaryotic Cells label cell,cell wall of peptidoglycan plasma membrane,cytoplasm w/ cytosol Ribosome,nucleoid w/ DNA draw three basic shapes determine stain reaction Gram positive = purple Gram negative = red

Models Study both animal and plant cell models