Revealing Glycoproteins in the Secretome from MCF-7 Human Breast Cancer Cells Aik-Aun Tan 1, Wai-Mei Phang 2, Subash C.B. Gopinath 4, Onn H. Hashim 5,

Slides:

Advertisements

Similar presentations

EXACT CALCULATION OF HIGGS PENGUIN VERTEX Swee-Ping Chia Physics Department, University of Malaya Kuala Lumpur, Malaysia

Advertisements

PROTEOMICS OF REDIVAC FLUID: COMPARATIVE ANALYSIS OF PROTEIN CLUSTERS IN PERITONEAL LAVAGE FLUID AND GYNAECOLOGICAL CANCER EUGENE LEONG WENG KONG 1, RAMARAO.

UNIVERSTI KEBANGSAAN MALAYSIA National University of Malaysia (UKM) The Guardian of The Nation Inspiring Future, Nurturing the Possibilities Prof Dr. Sarani.

Supplement Figure 1A. Representative 2D gel image of normal whey with 32 differently expressed protein spots identified by peptide mass fingerprinting.

Molecular Biology of the Cell

Evaluated Reference MS/MS Spectra Libraries Current and Future NIST Programs.

Molecular Biology of the Cell

Tryptic digestion Proteomics Workflow for Gel-based and LC-coupled Mass Spectrometry Protein or peptide pre-fractionation is a prerequisite for the reduction.

Mass spectrometry and mummies Professor Paul A. Haynes Biomolecular Frontiers Research Centre Department of Chemistry and Biomolecular Sciences Macquarie.

TEMPLATE DESIGN © Abstract Result 3 ： Statistical analysis of identified N α -acetylated peptides. Methods Conclusion.

Chapter 9 Mass Spectrometry (MS) -Microbial Functional Genomics 조광평 CBBL.

HERBALS IN PHARMACY: CHALLENGES Zhari Ismail PhD (U of Strathclyde) Pharmaceutical Chemistry Discipline School of Pharmaceutical Sciences Universiti Sains.

Article Title: AKT-aro and HER2-aro, models for de novo resistance to aromatase inhibitors; molecular characterization and inhibitor response studies Journal.

Conor Walsh Assistant Professor of Mechanical and Biomedical Engineering, SEAS Core Faculty Member, Wyss Institute for Biologically Inspired Engineering.

Supplement material to Ref. JASMS Ms. No Detection of an amine impurity and quality assessment related to this impurity of the UV MALDI matrix.

1 King Mongkut’s University of Technology Thonburi.

Biomedical Engineering at the Centre for Preclinical Research and Technology Contemporary medicine is challenged to seek methods for delaying and alleviating.

Graduate Program in Oral Biology M.S. and Ph.D.. Students are trained to become competent researchers with advanced knowledge and skills in research and.

Symptomatic Calcifying Pseudotumour of Thoracic Spine which Resolved with the Indomethacin Treatment. A Case Report. Kwan MK 1, Abdelhai AM 2, Saw LB 1,

Bio-Medical Engineer BY: Gabriel Cuilan. Bio-Medical Engineer Bio-Medical Engineers analyze and design solutions to problems in biology and medicine,

FACULTY of ARTS and SCIENCES HARVARD BUSINESS SCHOOL GRADUATE SCHOOL OF DESIGN HARVARD DIVINITY SCHOOL Center for the Study of World Religions Summer.

Copyright © 2012 Lippincott Williams & Wilkins. Unauthorized commercial reproduction of this slide is prohibited Supplemental PowerPoint Slides.

Spot number ; H1 Supporting Information Figure 3. MS spectra of the identified proteins.

Supplementary Figures. Figure Legends Suppl. Figure 1. Flow cytometry analysis to monitor apoptosis levels in MCF-7 cells. As control MCF-7 cells grown.

Molecular Biology of the Cell Fifth Edition Molecular Biology of the Cell Fifth Edition Chapter 21 Sexual Reproduction: Meiosis, Germ Cells, and Fertilization.

Molecular Biology of the Cell Fifth Edition Molecular Biology of the Cell Fifth Edition Chapter 25 The Adaptive Immune System Chapter 25 The Adaptive Immune.

2014 생화학 실험 (1) 6주차 실험조교 : 류 지 연 Yonsei Proteome Research Center 산학협동관 421호

Molecular Biology of the Cell

Figure Molecular Biology of the Cell (© Garland Science 2008)

Figure 14-1 Molecular Biology of the Cell (© Garland Science 2008)

Yonsei Proteome Research Center Peptide Mass Finger-Printing Part II. MALDI-TOF 2013 생화학 실험 (1) 6 주차 자료 임종선 조교 내선 6625.

Past, Present, & Future Research Dr. Helmi Norman Erasmus Mundus Postdoctoral Researcher Faculty of Science and Technology Bournemouth University.

Post translational modification n- acetylation Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying unknown protein. Proteins to.

Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling.

Department of Medical and Clinical Biophysics Faculty of Medicine Pavol Jozef Šafárik University in Košice, Slovakia Expertise in: Mass spectrometry based.

Supplementary Figure 1. Importance of first neighbours in breast cancer, hepatocellular carcinoma and non-small cell lung cancer in the SignaLink network.

Research interests Neurotoxicology of heavy metals

Bottom-Up Proteomics Data collection

Proteomic analysis of whole saliva from patients with oral cancer

Organoid Models and Applications in Biomedical Research

Electronic Supporting Information (ESI)

A b Suppl. Fig. 1.

Schematic representation of our multi-dimensional chromatography off-line MALDI-TOF analysis of the complex immunoproteome of class I molecules (A), and.

سرطان الثدي Breast Cancer

Data analysis of thermal proteome profiling

Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;4.A, MS/MS spectrum of singly phosphorylated 277ALGSFGSFGSFRSFA291 (m/z ).

Kenneth Song, Samir Hanash Gastroenterology

Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;7.A, MS/MS spectrum of singly phosphorylated 270ALGSFRSNATN280 (m/z ).

Innovative Design Contest 2018

Chapter 26 Proteomics: The Global Analysis of Proteins

Title of Research Poster. Line1 Line 2

Title of Research Poster. Line1 Line 2

Embryology in the era of proteomics

A A B C D Fraction number b MCF7 lysate fraction A B C D A B C D GST-p851 GST Kinase assay GST-p851.

Proteomic approach in the search of new cardiovascular biomarkers

Identification of SUMO3peptides from 2D-LC-MS/MS analyses of a tryptic digest of HEK293-SUMO3 cells using DDA and DIA methods. Identification of SUMO3peptides.

A, schematic presentation of fetuin-A domains.

Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.

Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,

Relative quantification of cis and trans PSP gp10040–42/47–52 variants

Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry. Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry.

Stability and variation of metrics over a range of sample injection amounts. Stability and variation of metrics over a range of sample injection amounts.

Schematic of AIMS-to-MRM experiment.

The Biological and Biomedical Joint Seminar Series

LABORATORY OF PROCESS ENGINEERING

MW (kDa) 2 µg 0.5 µg Supplementary Figure S1. SDS-PAGE under reducing condition analysis (Coomassie brilliant blue staining) of.

Navigating the Deubiquitinating Proteome with a CompPASS

Engineering Design Process

Figure 5-1 Molecular Biology of the Cell (© Garland Science 2008)

Presentation transcript:

Revealing Glycoproteins in the Secretome from MCF-7 Human Breast Cancer Cells Aik-Aun Tan 1, Wai-Mei Phang 2, Subash C.B. Gopinath 4, Onn H. Hashim 5, Lik-Voon Kiew 6 and Yeng Chen 2, 3* 1 Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang 11800, Malaysia 2 Department of Oral Biology & Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia 3 Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, Kuala Lumpur 50603, Malaysia 4 Institute of Nano Electronic Engineering (INEE) & School of Bioprocess Engineering, Universiti Malaysia Perlis, Kangar, Perlis, Malaysia 5 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia 6 Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia

(a) (b) Suppl. Fig. 1

(c) (d) Suppl. Fig. 1

(e) Suppl. Fig. 1

Supplementary figure legends S. Figure 1 Mass spectra of tryptic peptides derived from MCF-7 growth media. Protein identity of (a) carboxypeptidase A4 (CPA4), (b) alpha-1-antitrypsin (AAT), (c) haptoglobin (HP), (d) HSC-70 (HSC70), and (e) osteonectin (ON) were confirmed by ABI 4800 Proteomics Analyzer MALDI- TOF/TOF Mass Spectrometer. Analyses were performed in reflectron mode.