Garlic & Health Health Part University of Leipzig.

Slides:

Advertisements

Similar presentations

The Drug Discovery Process

Advertisements

2000 Consensus Statement "Dietary Fat, the Mediterranean Diet, and Lifelong Good Health" - London, January International Task Force for Prevention.

Onions- A Global Benefit to Health Gareth Griffiths, Laurence Trueman, Timothy Crowther, Brian Tomas and Brian Smith.

Monascus rice powder co-supplemented with coenzyme Q 10 improves serum lipids and LDL-lag time on hypercholesterolemia subjects. Shih-Wen Hsu 1#, Yi-Ting.

Pathogenesis of Atherosclerosis Judith Berliner, Ph.D. Departments of Pathology and Medicine Division of Cardiology David Geffen School of Medicine at.

Marie-Hélène SIESS et al.

Effect of garlic on inflammation processes and atherosclerosis under human-like conditions in APOE*3-Leiden mice Sonia M.S. Espirito Santo TNO Prevention.

Garlic & Health WP 5.2 Cholesterol biosynthesis Frank Struck, Doris Kellert Dr. Sebastian Zellmer, Dr. Gayane Buniatian.

Cholesterol. The Good the Bad the Ugly.

Cholesterol and C.V.D.. Learning outcome Most cholesterol is synthesised by the liver from saturated fats in the diet. Cholesterol is a component of cell.

Free radicals and antioxidants in health and disease

ATHEROSCLEROSIS. Atherosclerosis Atherosclerosis is a disease of large and medium-sized muscular arteries and is characterized by endothelial dysfunction,

Lipoprotein Structures, Function and Metabolism (4)

WP 6 UMR de Toxicologie Alimentaire INRA Dijon WP 6 Cancer : General objectives  inhibition of bio-activation of carcinogens  stimulation of detoxification.

WP 6 UMR de Toxicologie Alimentaire INRA Dijon WP 6 Cancer : General objectives  inhibition of bio-activation of carcinogens  stimulation of detoxification.

1 Human Intervention Study From Report of the EU reviewers (Midterm review , Brussels) “The human studies need careful thought otherwise there.

Garlic & Health:Health part - general aim Main Goal:- Interactive improvement of garlic quality Elucidation of mechanism Analysis of active of action compoundsImproved.

Effects on lipid levels and lipid metabolism under more human-like conditions. –Lipid levels, cholesterol synthesis and cholesterol absorption. Effects.

Copyright® Raisio A plant stanol yogurt drink alone or combined with a low-dose statin lowers serum triacylglycerol and non-HDL cholesterol.

1 European Union Garlic Inflammation Study (EUGIS) History Garlic would influence lipid metabolism / atherosclerosis Proposed study: - 2 x 24 apparently.

EUGIS European Union Garlic Inflammation Study in Humans.

Rolf Gebhardt University of Leipzig Medical Fakulty Institute of Biochemistry Garlic & Health: WP 5.2 The interaction of Garlic with vessel wall and cholesterol.

Garlic & Health a European initiative for the development of high quality garlic and its influence on biomarkers of atherosclerosis and cancer in humans.

Effect of garlic on inflammation processes and atherosclerosis under human-like conditions in APOE*3-Leiden mice Sonia M.S. Espirito Santo TNO Prevention.

Main Topics of Research in the Health Part Effects of garlic powders and known compounds on cellular functions related to arteriosclerosis or inflammation.

Rolf Gebhardt University of Leipzig Medical Fakulty Institute of Biochemistry Garlic & Health: WP 5.2 The interaction of Garlic with vessel wall and cholesterol.

Biomarkers Biomarkers - markers in biological systems with a sufficently long half-life which allow location where in the biological system change occur.

Sonia M.S. Espirito Santo, Bart J.M. van Vlijmen, Rien Buytenhek, Wim van Duyvenvoorde, Erik H. Offerman, Louis M. Havekes and Hans M.G. Princen Gaubius.

EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF GARLIC SULFUR COMPOUNDS IN HEPG2 CELLS C. Belloir, M.H. Siess, C. Daurat and A.M. Le Bon INRA, UMR Toxicologie.

Copyright® Raisio Effects of long-term plant sterol or stanol ester consumption on lipid and lipoprotein metabolism in subjects on statin treatment.

Rolf Gebhardt University of Leipzig Medical Fakulty Institute of Biochemistry Garlic & Health: WP 5.2 Effect of Garlic on Cholesterol Biosynthesis.

WP 6 UMR de Toxicologie Alimentaire INRA WP6 : Effects of garlic on carcinogen metabolizing enzymes Milestones :  In vitro effects of garlic compounds.

Aorta Adhesion Molecules in Cholesterol-fed Rats Supplemented with Extra Virgin Olive Oil or Sunflower Oil, in Either Commercial or Modified Forms Ageliki.

European Union Garlic Inflammation Study (EUGIS) in Humans Martijn van Doorn Sonia Espirito Santo TNO-PH Leiden The Netherlands CHDR Leiden The Netherlands.

L-Arginine Clinical Trial Results of Study 18% increase in HDL 18% increase in HDL 40% decrease in triglycerides 40% decrease in triglycerides 8% glucose.

 Prof. A. M. Vollmar  PD Dr. V. M. Dirsch  Thomas Roos  Nancy Lopez WP 5: Cardiovascular diseases 5.1: The Interaction of garlic with mediators of.

Cardiovascular Disease (CVD) A general term for diseases of the heart and/or blood __________ _____% of all deaths annually 2,600 American deaths each.

Rolf Gebhardt University of Leipzig Medical Fakulty Institute of Biochemistry EU-Project: Garlic & Health Effects of High Quality Garlic on Markers of.

1 G&H Meeting 4-5 th October, 2001 G&H Meeting 4-5 th October, 2001 Wellesbourne, UK Wellesbourne, UK WP 7 Pharmaceutics WP 7 Pharmaceutics Objectives.

STUDY OF ANTIHYPERLIPIDEMIC ACTIVITY OF SOME MEDICINAL PLANTS.

Protection of garlic against carcinogen genotoxicity Anne-Marie Le Bon, Christine Belloir, Varsha Singh Milestones :  In vitro evaluation of the antigenotoxic.

Cardiovascular Disease

Better Health. No Hassles. HIGH BLOOD CHOLESTEROL Cholesterol is found in every cell in our body. Cholesterol is used to build healthy cells, as well as.

Bioavailability of Dietary Supplements: Key Issues in Defining the Research Agenda Impact of Formulation on Bioavailability? Discussion Leader: Stephen.

Extracts from garlic dry powders modulate LPS-induced liberation of cytokines. H.-P. Keiß 1, V.M. Dirsch 1, T. Hartung 2, A.M. Vollmar 1 1 Department of.

ABOUT OUR NEW HPLC METHOD  We have identified a new compound in garlic HPLC profile, the S-allyl cystein (SAC) that has a potential therapeutic activity.

The Interaction of Garlic With Mediators of Inflammation Prof. A. M. Vollmar Dr. V. M. Dirsch H - P Keiß.

European Patients’ Academy on Therapeutic Innovation Non-clinical development.

The studied molecules are : Range : 5 to 100 µMDADS (diallyl sulfide) DADSO (allicin) SAC (S-allyl cystein) AM (allyl mercaptan) Anti-genotoxicity studies.

GARLIC AND HEALTH in vivo Metabolism of DADS 4 th October 2001 Study of in vivo Diallyl disulfide Metabolism Aims of the study : –To determine the pharmacokinetic.

Emmanuelle Germain, Joelle Chevalier, Marlène Steib, Marie-Hélène Siess* and Caroline Teyssier* Institut National de la Recherche Agronomique / ENSBANA,

Lab 6 TC, LDL-C, HDL-C, and TG T.A. Bahiya Osrah

What do I remember? What is thrombosis?

The Role of Cell Adhesion in Inflammation and Metastasis 赵燃丁合

Health Benefits PHTYOME FP7-SME PHYTOME (315683) Brussels, November 16, 2015 Prof. dr. Theo M. de Kok Coordinator PHYTOME project Maastricht University,

Lipoprotein Structure, Function, and Metabolism

Cardiovascular Disease

Drug Discovery &Development

Rambam health care campus, Haifa, Israel

M.Sc. in Pharmacy/Clinical Laboratory Sciences

Fig. 1. Serum total cholesterol (A), LDL cholesterol (B), triglyceride (C) and HDL cholesterol level (D) according to HBsAg status in age group. Total.

Tirawanchai N , Somkasetrin A , Kengkoom K, Ampawong S

Lorena Bouzas Alfonsín Advanced Genetics

R Keith Randolph PhD Technology Strategy Nutrilite Health Institute

Chapter 10 Diet and Health

Ch 13.6: Blood Vessels 13.7: Athrosclerosis and Cardiac Arrhythmias

Advanced Nutrition Diet and Cardiovascular Disease 1

Figure 2 Protective functions of HDL

Atherosclerosis Christopher K. Glass, Joseph L. Witztum Cell

Positive Genetox Findings on a Candidate Pharmaceutical…. Now What

Presentation transcript:

Garlic & Health Health Part University of Leipzig

Garlic & Health:Health part - general aim University of Leipzig Main Goal:- Interactive improvement of garlic quality Elucidation of mechanism Analysis of active of action compoundsImproved drug formulation High quality garlic Improvement of garlic Optimal use of garlic plants and growth conditionsfor therapeutic application Main Focus:- Inflammation and cytokine signaling - Atherosclerosis and serum cholesterol - Cancer and biotransformation - Human Intervention Study

Garlic & Health: WP5 Cholesterol and cytokines University of Leipzig High Quality Garlic: Inhibition of cholesterol biosynthesis Indirect Mechanism of Inhibition: Aim:Interaction of garlic constituents with cholesterol biosynthesis

Garlic & Health: WP5 Cholesterol and cytokines University of Leipzig Challenge for future project research : Plant part:Health part: Definition of growth conditionsAnalysis of responsibel compounds FertilisationElucidation of mechanism of action (Genetic) Modification of plantsImplications for human health Unexpected Result !! Low Quality Garlic: Stimulation of cholesterol biosynthesis !

Garlic & Health: WP5 Cholesterol and cytokines University of Leipzig Aim:Interaction of garlic constitutents with vessel wall Inhibition of Matrix Metalloproteinase-2 Production by DADS, AM and SAC in HCA- endothelial cells No influence on adhesion molecules and NF-kB activity

Garlic & Health: WP5 Cholesterol and cytokines University of Leipzig Aim:Interaction of garlic constitutents with cytokine production in human blood Garlic extracts influence the liberation of pro- and antiinflammatory cytokines in human blood High quality garlic shows a better biological cytokine profile Cytokine modulation may indirectly affect NF-kB activation

Garlic & Health: WP6 Cancer and biotransformation University of Leipzig Aim:Bioavailability of garlic constituents DADS DADSO In vivo AGS SH AM AMS AMSO2 AMSO S glutathione In vitro

Garlic & Health: WP6 Cancer and biotransformation University of Leipzig Aim:Protection against genotoxicity by garlic constituents % of DNA alterations H202H202 5 µM25 µM50 µM100µM Effects of S-allylcystein on H 2 O 2 genotoxicity in HepG2 cells Effects of garlic powders on hepatic UGT activity in rat consuming garlic powders Aim:Modulation of enzymes of carcinogen activation or detoxification Fertilization

Garlic & Health: WP7 Pharmaceutics University of Leipzig Aim:- Development of reliable analytical methods - Development of improved pharmaceutical formulation „All purpose“ HPLC method Comparison European Market Products

Garlic & Health: WP8 Human Intervention Study University of Leipzig Aim:Placebo-controlled study Run-in periodIntervention period weeks Blood and urine collection Healthy volunteers with increased risk (mildly hyercholesterolemic) Surrogate markers of atherosclerosis: Plasma lipids (cholesterol, triglycerides, HDL, LDL, lp(a)) Von Willebrand factor Adhesion molecules (VCAM-1, ICAM-1, E-selectine) Surrogate markers of cancer: Phase II enzymes DNA damage