Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007
University of MN Department of Horticulture
2,4-dichlorophenoxyacetic acid (2,4-D)
Background Light intensity and hue angle decreases (Rosen, et al.)
Components of Color Light intensity Chroma (saturation) Hue angle
Components of Color Light intensity Chroma (saturation) Hue angle
Components of Color Light intensity Chroma (saturation) Hue angle
Components of Color Light intensity Chroma (saturation) Hue angle
Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.)
Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.) 2,4-D harmful, LD50 of 1500 ppm ( MSDS found at )
Goal To determine visual color, anthocyanin accumulation, and UFGT accumulation differences
Anthocyanin Biosynthetic Pathway LDOX UFGT leucocyanidin anthocyanidin anthocyanin 3-glucoside (Keifenheim, et al.)
Hypotheses With 2,4-D application: Increase in UFGT accumulation
Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.)
Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.) Increase in anthocyanin accumulation
Methods
Colorimetry
Components of Color Light intensity Chroma (saturation) Hue angle
Anthocyanin Accumulation
Quantifying UFGT RNA
Quantifying UFGT cDNARNA reverse transcription
Quantifying UFGT cDNA Amplified UFGT RNA reverse transcription qPCR
Results SS
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study
Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study
UFGT Field Data
UFGT Greenhouse Data
Conclusion Visual color significantly changed (light intensity, hue angle)
Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged
Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged UFGT accumulation decreased
Future Work Look at petunidin (purple) vs. pelargonidin (red) anthocyanins
Acknowledgements Dr. Cindy Tong Harpartap Mann Dr. Carl Rosen Ms. Fruen Team Research
Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007