Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007.

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Presentation transcript:

Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007

University of MN Department of Horticulture

2,4-dichlorophenoxyacetic acid (2,4-D)

Background Light intensity and hue angle decreases (Rosen, et al.)

Components of Color Light intensity Chroma (saturation) Hue angle

Components of Color Light intensity Chroma (saturation) Hue angle

Components of Color Light intensity Chroma (saturation) Hue angle

Components of Color Light intensity Chroma (saturation) Hue angle

Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.)

Background Light intensity and hue angle decreases (Rosen, et al.) Coloration affects sales (Fults, et al.) 2,4-D harmful, LD50 of 1500 ppm ( MSDS found at )

Goal To determine visual color, anthocyanin accumulation, and UFGT accumulation differences

Anthocyanin Biosynthetic Pathway LDOX UFGT leucocyanidin  anthocyanidin  anthocyanin 3-glucoside (Keifenheim, et al.)

Hypotheses With 2,4-D application: Increase in UFGT accumulation

Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.)

Hypotheses With 2,4-D application: Increase in UFGT accumulation Change in color (Rosen, et al.) Increase in anthocyanin accumulation

Methods

Colorimetry

Components of Color Light intensity Chroma (saturation) Hue angle

Anthocyanin Accumulation

Quantifying UFGT RNA

Quantifying UFGT cDNARNA reverse transcription

Quantifying UFGT cDNA Amplified UFGT RNA reverse transcription qPCR

Results SS

Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

Light Intensity, Chroma, Hue Angle, Anthocyanin Accumulation Field StudyGreenhouse Study

UFGT Field Data

UFGT Greenhouse Data

Conclusion Visual color significantly changed (light intensity, hue angle)

Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged

Conclusion Visual color significantly changed (light intensity, hue angle) Anthocyanin accumulation unchanged UFGT accumulation decreased

Future Work Look at petunidin (purple) vs. pelargonidin (red) anthocyanins

Acknowledgements Dr. Cindy Tong Harpartap Mann Dr. Carl Rosen Ms. Fruen Team Research

Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007