FT-ICR MS Fourier transform ion cyclotron resonance Mass spectrometer.

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Presentation transcript:

FT-ICR MS Fourier transform ion cyclotron resonance Mass spectrometer

Dr. Alan G. Marshall Invented in 1974 (The University of British Columbia) Ohio State University Florida State University (Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory) FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY: A PRIMER Alan G. Marshall, Christopher L. Hendrickson, and George S. Jackson (1998) Mass Spectrometry Reviews 17, 1-35

Pacific Northwest National Laboratory - (Dr. Richard Smith) University of Illinois (Dr. Neil Kelleher - Northwestern University) Commercial instruments in many other institutes and universities

F is the Lorentz Force observed by the ion when entering the magnetic field B is the magnetic field strength (constant) v is the incident velocity of the ion w c is the induced cyclotron frequency m is the mass of the ion z is the charge on the ion Condensed Theory of FT-MS Ions generated at the ionization source are introduced into the mass spectrometer by the use of high vacuum pump systems By the time ions enter the ion trap (cell) : vacuum mBar, temperature absolute zero The cell is located inside a spatially uniform superconducting magnetic field of usually between 4 – 15 Tesla cooled by liquid Helium and liquid Nitrogen Ions are bent into a perpendicular circular path by the magnetic field RF applied to ions Ions with the same frequency with the RF are recorded, their intensity is proportional to the concentration of a particular ion

Diagramatic Scheme of ions in a FT MS – (Taken from U of Bristol, UK)

ICR Cell

Linear Ion Trap Mass spectrometer with FT-ICR detector