You should be able to label these pictures Label the following: –RNA polymerase –DNA –mRNA –tRNA –5’ end –3’ end –Amino acid –Ribosome –Polypeptide chain –Codon –anticodon
Focus Question: How does transcription work in prokaryotes and eukaryotes? IB Topic 7.3 Transcription
The “Players” DNA RNA polymerase mRNA Ribosome tRNA Amino acids
Definition Transcription- the reading and recording of DNA code. Location: Nucleus
Direction of Transcription Occurs in the 5’ 3’ direction (with reference to the mRNA)
IB Terms: Sense vs. Antisense strands Sense strand- the “other” strand, not transcribed. Antisense strand- the one mRNA attaches to, and is complimentary to. mRNA is similar to the sense strand, except T U.
New…the “Middle” Step Transcription- beginning Middle = mRNA processing Translation- end
RNA processing Post-transcriptional Introns- non-coding sequences are removed Exons- coding sequences are spliced together
New Players: Prokaryotic Transcription Promoter- place on gene where RNA polymerase initially attaches. Terminator- place on gene where transcription ends.
RNA Nucleotides = Ribonucleoside Triphosphates Similar to deoxynucleoside triphosphates. Serve as the building blocks for mRNA. Can work from 5’ to 3’ OR 3’ to 5’