Biological Drawings and Microscope Calculations Examining Protists

Parts of the Microscope

Microscope Calculations 1. Total Magnification = magnification of eyepiece x magnification of objective lens ocular lens = 10X low power = 4X med power = 10X high power = 100X Low TM = Med TM = High TM =

2. Measuring Field of View on Low Power use low power place clear plastic ruler with mm markings on top of the stage focus your image count how many divisions of the ruler fit across the diameter of the field of view multiply the number of divisions by 1000 to obtain the field of view in micrometers (µm) record this in µm (1mm = 1000 µm ). 2 mm x 1000 = 2000 µm3 mm x 1000 = 3000 µm

3. Measuring FOV on other powers ex. if at low power FOV is 2000µm, calculate FOV for: 1. med power 40X = __?___ 100X 2000µm ? = (40X x 2000µm) 100X = 800µm 2. high power 40X = __?___ 400X 2000µm ? = (40X x 2000µm) 400X = 200µm

4. Estimating Actual Size estimate the length of the amoeba based on the diameter of the field of view ex FOV = 750 µm amoeba = 40% FOV diameter = 300µm

5. Magnification of the Drawing how many times the drawing has been magnified Magnification of Drawing = drawn size (µm) actual size (µm) =100, 000 µm (100 mm x 1000) 300 µm = X

Making Biological Drawings 3_HOW TO MAKE BIOLOGICAL DRAWINGS.pdf 3_HOW TO MAKE BIOLOGICAL DRAWINGS.pdf