Calculating Cell Size.

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Presentation transcript:

Calculating Cell Size

Key Terms Resolution: the ability to distinguish two separate points as distinct from each other Magnification: the number of times greater an image is than the object

Practicing magnification maths There are: 1000 millimetres in a metre (mm) 1 000 000 micrometres in a metre (mm) 1 000 000 000 nanometres in a metre (nm)

How many micrometres are 10mm? 8mm? 17mm? 1cm? 2.5cm? 13cm? 29cm? 14cm? 0.2cm? 1m?

Calculating Actual Size Actual size = image size magnification Magnification = image size actual size Worksheet

Eye Piece Graticule Microscopes can be fitted with an EPG Ruler etched on it A specimen can be measured in eyepiece units (an arbitrary measurement, the image changes size depending on the magnification, it needs to be calibrated)

Using Stage Micrometer Place a microscope ruler on a slide (micrometer) on the stage The ruler is 1mm long and split into 100 divisions Each division is 10µm (0.01mm) 1µm is equal to 1 millionth of a metre

Calculating size In the image (a), the nucleus is 3.2epu With x100 mag. 1epu = 10mm So: the nucleus is 3.2x10 = 32mm Look at your graticule and stage Micrometer. Use them to calculate the size of an onion cell and a cheek cell.

Plenary Question If a nucleus measures 100mm on a diagram, with a magnification of x10000, what is the actual size of the nucleus?