Microbiologic Staining Method

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Presentation transcript:

Microbiologic Staining Method 顏慕庸高雄榮總

Microbiological Staining Gram stain Acid fast stain KOH stain Wet mount Indian ink

Gram Stain Christian Gram, 1884 crystal violet iodine-KI alcohol wash safranin

Significance Culture Buy time for golden period Diagnosis

Interpretation Morphological Associate environment Site-specific Immunity-oriented

Morphologic interpretation cocci vs bacilli, cocco-bacilli Gram positive vs Gram negative

Morphologic interpretation cocci vs bacilli, cocco-bacilli Gram positive vs Gram negative

Gram-Positive Cocci GPC in group GPC in chain GPC w/c capsule GPC in pair lancet shape kidney shape sarcinae

Gram-Positive Cocci GPC in group GPC in chain GPC w/c capsule GPC in pair lancet shape kidney shape sarcinae

Cocco-bacilli, Kidney-shape G (+) vs G (-) Gram Negative Cocci Cocco-bacilli, Kidney-shape G (+) vs G (-)

Bacilli Gram positive bacilli spore spindle box car palisade beaded branching Gram negative bacilli pleomorphic cocco-bacilli capsule curved

Bacilli Gram positive bacilli spore spindle box car palisade beaded branching Gram negative bacilli pleomorphic cocco-bacilli capsule curved

Presumptive Diagnosis Staphylococcus aureus Streptococcus pneumoniae Hemophilus influenzae Klebsiella pneumoniae Nocardia spp Candida spp

Associate environment Neutrophils, epithelial cells Aid for judgement of G(+), G(-) Intracellular vs extracellular Mixed flora w/o PMN, w/o bacteria Non-bacteria organisms

Associate environment Neutrophils, epithelial cells Aid for judgement of G(+), G(-) Intracellular vs extracellular Mixed flora w/o PMN, w/o bacteria Non-bacteria organisms

Associate environment Neutrophils, epithelial cells Aid for judgement of G(+), G(-) Intracellular vs extracellular Mixed flora w/o PMN, w/o bacteria Non-bacteria organisms

Site specific Gram negative cocci urine: CSF: sputum: Gram positive bacilli CSF: sputum: Gram positive cocci urine:

Site specific Gram negative diplococci urine: N. gonorrheae CSF: N. meningitidis sputum: M. catarrhalis

Site specific Gram positive bacilli CSF: L. monocytogenes sputum: Corynebaterium straitum Gram positive cocci urine: Enterococcus , Viridans strep.

Immunity - oriented Nosocomial infection Community-acquired GNB in CSF cocco-bacilli in sputum T-cell immunity GPB in CSF Community-acquired GNB in CSF cocco-bacilli in sputum T-cell immunity GNB in joint

Immunity - oriented Community-acquired GNB in CSF K. pneumoniae cocco-bacilli sputum H. influenzae Nosocomial infection GNB in CSF P. aeruginosa Enterobactericeae cocco-bacilli in sputum A. baumannii

Immunity - oriented T-cell immunity T-cell immunity GPB in CSF L. monocytogenes T-cell immunity GNB in joint Salmonella spp.

Acid Fast Stain Ziehl-Neelsen stain Kinyoun modification Mycobacteria Nocardia, partial stain NOT for actinomycosis