THE IMPORTANCE OF LABELLING YOUR S(TUFF) In the Globe and Mail this weekend there was a little anecdote that went as follows: PhD student spends three years in tropical rainforest laboriously collecting lizard excrement for some sort of analysis. The PhD student stores the excrement in the lab. freezer in an unlabelled plastic bag. The PhD student returns to rainforest to collect more excrement (apparently this stuff is difficult to get!) The PhD student returns home. Finds out all his excrement has been thrown out in a lab clean-up. PhD student says his career and life are ruined. Let this tragedy be a warning to us all.

THE RER/GOLGI SYSTEM

The Nobel Prize in Physiology or Medicine "for their discoveries concerning the structural and functional organization of the cell" George Palade Christian de Duve INTRACELLULAR PATHWAY OF SECRETORY PROTEIN TRAFFIC LYSOSOMES/PEROXISOMES

Charles Leblond, McGill University Marian Neutra, Harvard Medical School ROLE OF THE GOLGI BODY

Palade is credited with being the first one to describe the small particles that later came to be known as ribosomes.

HOW DID PALADE AND CO-WORKERS ELUCIDATE THE PATHWAY OF SECRETORY PROTEIN TRAFFIN IN CELLS? HOW DID NEUTRA AND LEBLOND ELUCIDATE THE ROLE OF THE GOLGI BODIES IN PPROTEIN SECRETION?

CHOOSE THE BEST CELLS TO WORK WITH! PALADE AND CO-WORKERS DIGESTIVE CELLS OF PANCREAS NEUTRA AND LEBLONDSMALL INTESTINE

FIBROBLAST OSTEOCYTE

(2) USE THE BEST TECHNIQUES PALADE AND CO-WORKERS DIGESTIVE CELLS OF PANCREAS NEUTRA AND LEBLONDSMALL INTESTINE (1) CHOOSE THE BEST CELLS TO WORK WITH! MICROAUTORADIOGRAPHY

DUCT ZYMOGEN GRANULES MITOCHONDRIA ACINAR CELLS ROUGH ENDOPLASMIC RETICULUM BASEMENT LAMINA DUODENUM

β-PARTICLES 3 H HELIUM + β-PARTICLE

USE THE AMINO ACID LEUCINE IN WHICH AN R-GROUP 1 H-ATOM HAS BEEN REPLACED WITH AN 3 H-ATOM

SECRETORY VESICLES SECRETORY VESICLES WITH PROTEIN MADE DURING THE EXPERIMENT

DECAY TRACK OF ONE β-PARTICLE

PULSE-CHASE EXPERIMENT

Charles Leblond, McGill University Marian Neutra, Harvard Medical School ROLE OF THE GOLGI BODY

WANTED TO STUDY ROLE OF GOLGI BODY USED 3 H-FUCOSE (A SUGAR) INSTEAD OF 3 H- LEUCINE (AN AMINO ACID)

GOBLET CELLS FROM EPITHELIUM OF SMALL INTESTINE

GOBLET CELLS OF RAT INTESTINE

THE MECHANISM OF POLYPETIDE IMPORT INTO THE RER

PAGE 697Post-translational import

MELANOSOMES CENTRAL VACUOLE INTEGRAL MEMBRANE PROTEINS P 687 Co-translational import

GUNTHER BLOBEL SIGNAL HYPOTHESIS