Topic 7: Cellular Genetics. Exercise II Mitosis Cell Cycle Exp: Cell cycle onion tips Topic: DNA Exercise I: Isolation Analysis Plasmid DNA.

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Presentation transcript:

Topic 7: Cellular Genetics. Exercise II Mitosis Cell Cycle Exp: Cell cycle onion tips Topic: DNA Exercise I: Isolation Analysis Plasmid DNA

Onion root tip.

Structure of bacteria.

Two strains of bacteria: pBR322 and pUC18

Isolation of Plasmid DNA: 1. Collect bacteria.

Isolation of plasmid DNA: 2. Add:

Isolation of Plasmid DNA: 3. Precipitate proteins, chromosomal DNA, and some of the RNA.

Isolation of plasmid DNA: Note: Addition of very small quantities of liquids.

Isolation of plasmid DNA: 4. DNAse and RNAse treatments.

Agarose Gel.

Electrophoresis:

Isolation of Plasmid DNA.

pBR322 pUC18 ET D R

pUC18pBR322 ET D R ET R D