AP Biology Biotechnology Part 2 Genetics of Bacteria.

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Presentation transcript:

AP Biology Biotechnology Part 2 Genetics of Bacteria

Bacterial “size”

Prokaryote Structure

Bacterial genome

Theta Replication Origin of replication Replication fork Termination of replication

Transformation

Transduction A+A+ Phage DNA A+A+ Donor cell B+B+ A+A+ B+B+ Crossing over A+A+ A–A– B–B– Recipient cell A+A+ B–B– Recombinant cell

. Sex pilus 5 µm

DNA transfer “Conjugation”

Barbara McClintock Maize

Transposon -Composite Transposon Insertion sequence Insertion sequence Antibiotic resistance gene Transposase gene Inverted repeat

Transposons

Operon Regulation Promoter DNA trpR Regulatory gene RNA polymerase mRNA 3 5 Protein Inactive repressor Tryptophan absent, repressor inactive, operon on mRNA 5 trpE trpD trpC trpBtrpA Operator Start codon Stop codon trp operon Genes of operon E Polypeptides that make up enzymes for tryptophan synthesis D C B A

Repressor Operon

Operon Repressor DNA Protein Tryptophan (corepressor) Tryptophan present, repressor active, operon off mRNA Active repressor No RNA made

Inducer Operon

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