Lecture Title: Fungi and their pathogenesis

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Presentation transcript:

Lecture Title: Fungi and their pathogenesis (Foundation Block, Microbiology)

Lecture Objectives.. To describe the general characteristics of fungi and recognize a fungus from all other living organisms To establish familiarity with the terminology needed by medical students To know certain fundamental facts about classification reproduction and identification of fungi

What is Mycology?.. Mycology: Study of fungi Kingdom myceteae (= Kingdom fungi) Medical mycology : Study of medically important fungi and the mycotic diseases. Mycoses: A disease caused by a fungus

What is a Fungus ? Characteristics of fungi: All Eukaryotic organisms (a true nucleus) Heterotrophic (Saprobic, symbiotic, parasitic) Do not have chlorophyll (Achlorophyllous) The cell is surrounded by rigid cell wall made of chitin and complex carbohydates (Mannan, glucan) Cell membrane : (sterol, ergosterol)

What is a Fungus ? Characteristics of fungi Saprobic Parasitic feed on dead tissues or organic waste (decomposers) Symbiotic mutually beneficial relationship between a fungus and another organism Parasitic feeding on living tissue of a host. (disease)

MORPHOLGY Yeasts : are unicellular organisms Filamentous fungi (Hyphae, mycelium) Hyhpae are multicellular filamentous structures, constituted by tubular cells with cell walls. Dimorphic Yeast : Parasitic form, Tissue form, Cultured at 37° C Filamentous : Saprophytic form, Cultured at 25 C Dimorphic: Have two forms depending on change in the environmental factors Mold form Yeast form

How do we differentiate between them? MORPHOLGY Yeast: Colony morphology (Culture) Have same appearance How do we differentiate between them? Budding yeast cell In Clinical samples Budding yeast cells +/- Pseudohyphae Examples : Candida albicans, Saccharomyces cerevisiae

MORPHOLGY Filamentous fungi (Mould=Mold) A hypha (plural hyphae) is a long, branching filamentous cell. hyphae are the main mode of vegetative growth. Mycelium: The intertwined mass of hyphae that forms the fungal colony. Conidia/ Spore: asexual spores borne externally on hyphae or on a conidiophore. Examples: Aspergillus, Penicillium, Rhizopus

Filamentous fungi Hyphal growth from spore MORPHOLGY Filamentous fungi Hyphal growth from spore Spore/ conidia mycelium

MORPHOLGY Filamentous fungi Colonial morphology Penicillium, Rhiaopus

MORPHOLGY Filamentous fungi Conidia / spore : asexual spores borne externally on hyphae or on a conidiophore. Conidia

Fungal Hypha Filamentous fungi Septa: Cross-walls (septa) that divide hyphae into segments. (septate hypha) If there are no cross-walls, the hyphae are considered to be non-septate.

MORPHOLGY Filamentous fungi (mold) Moniliaceous molds hyaline or lightly pigmented conidia or hyphae, colorless Dematiaceous Molds Are pigmented. Because of the pigment, the colonies appear dark, brown, or black

Reproduction in Fungi I) Asexual: Only mitotic cell division 1) Somatic Yeasts by budding Molds by hyphal fragmentation 2) Spore formation: II) Sexual: Fusion, mitosis, meiosis

Spores? These are the small airborne particles by which fungi reproduce. They are produced by mitosis and readily disseminate in the air. SPORES

PATHOGENICITY OF FUNGI Fungi can cause diseases to humans Cause superficial infections, some can cause allergic reactions Few cause invasive infections Not all fungi are pathogenic To cause the disease: Thermotolerance Ability to survive in tissue environment Ability to withstand host defenses

Thank You  (Foundation Block, Microbiology)