Feb 3, 2015 I can… Predict how DNA is used to solve real- world problems Reminders: February 4 th – All first semester work must be turned in, please check.

Slides:

Advertisements

Similar presentations

Ch. 2A: How Do You Begin to Clone a Gene?. Learning goals Describe the characteristics of plasmids Explain how plasmids are used in cloning a gene Describe.

Advertisements

Stage 1: Prepare your plasmids to be cut by restriction enzymes Obtain the plasmids (pKAN and pAMP) P stands for plasmid pKAN = plasmid with antibiotic.

Gel Electrophoresis Lab Analysis of Precut Lambda DNA.

Standard Using lab techniques, such as DNA extraction, PCR, restriction enzymes and gel electrophoresis to determine the presence or absence of a SNP in.

BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.

Restriction Digest Laboratory. Reminder You have transformed bacteria with plasmid DNA You have isolated plasmid DNA Today you will perform an RFLP analysis.

Discussion Questions youtubeprotein synthesis codon chart is on pg 338

13-2 Manipulating DNA.

Unit 13 Biotechnology. The use of cells and biological molecules to solve problems or make useful products Involves manipulation of DNA and RNA.

DNA is made of Nucleotides (p. 282). Nitrogen Bases.

Unit 8 test Biotech study guide.

Unit 8 Vocabulary 1. micropipette- tool used to transfer small volumes of liquid 2. P 20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used.

6th Grade Science 6th Grade Science Topics (PA State Standards & TE Essential Skills) Books (Class copy and online text book) Earth’s Waters Cells and.

6 th Grade Science 6 th Grade Science Topics (PA State Standards & TE Essential Skills) Books (Class copy and online text book) Earth’s Waters Cells and.

Restriction Analysis and Digestion of Lambda DNA.

AP Biology DiscussionNotes Friday 01/16/2015. Goals for Today Be able to describe how restriction enzymes work and why they are valuable to scientists.

Warm-up : 4/4/11 Complimentary Strand Do this on a separate sheet in your notebook. What pairs up with: –Adenine? –Guanine? For the following strands give.

Unit 6: DNA Grab a new Warm Up!. Monday 4/13 Learning Targets: 1) I can work with my lab team to create a presentation of my experimental research. 2)

What are Plasmids? Plasmids are circular pieces of bacterial DNA that often contain genes not related to basic life functions Often contain antibiotic.

Restriction Enzyme Cleavage of DNA

Using a Single-Nucleotide Polymorphism to Predict Bitter- Tasting Ability Carolina Kit.

Warm-Up #33 Answer questions #1-5 on Text page 321, Section Assessment.

Wildlife Forensics Environmental Study Center. Chinchilla.

Program Introduction Chapter 1 – Some Tools of the Trade.

Oct 6, 2014 I can… Describe my initial ideas about animals Record initial data for plant investigation Homework: Food Molecule Quiz 10/22 Animal Test 10/29.

Do Now 12/7 1.Copy down this week’s homework 2.Put your Scientific Explanation rubric in the In Box (make sure your name is on it!). 3.Turn to NB p53 MondayTuesdayWednesdayThursdayFriday.

Section 14-3: Studying the Human Genome. Manipulating DNA The SMALLEST human chromosome contains 50 million bases DNA is a HUGE molecule that is difficult.

Restriction Digest Laboratory Restriction fragment length polymorphism.

Discussion Questions Discussion Questions 10/27/15 1. Using these words, please describe DNA Replication: semi-conservative, unzips, Helicase, DNA Polymerase,

Warm-up 12/2/15: Write the questions and the answers to the questions on a new warm-up sheet for the week. Where is DNA found in eukaryotic plant and animal.

DNA REPLICATION Chapter 11, Section 1. DNA Review What is the building block of DNA? Nucleotides What is the shape of DNA? Double Helix What holds together.

Vocab review Unit 8 - biotechnology. 1. Organism that has acquired genetic material by artificial means.

Inquiry 2/8/16 No warm-up today! Take out something to write with for our DNA Quiz. Put your lab book on the lab station by the fume hood. If you finish.

AGENDA – 11/5/15 Take out journal! DNA and Cell Cycle Review Game Finish Exam Study Guide Homework: TEST TOMORROW! Exam Study Guide due tomorrow! JOURNALS.

DNA TO PROTEIN Biology - 9. Monday, Nov. 25 th  Objective: Students will put the vocabulary of DNA and protein synthesis into context.  Go Over Test.

VycToLHVnHs The Suspects 1.Ms. Hall 2.Clarence 3.Eduardo (Gertrude’s Boyfriend) 4.Britney (Gertrude’s best friend) 5.The.

DNA Forensics 352 – O’Dette. Why DNA? DNA is individual evidence DNA links or eliminates a suspect to a crime DNA identifies a victim even if no body.

DNA Fingerprinting Review. Why DNA? DNA is individual evidence DNA links or eliminates a suspect to a crime DNA identifies a victim even if no body is.

Standard Using lab techniques, such as DNA extraction, PCR, restriction enzymes and gel electrophoresis to determine the presence or absence of a SNP in.

Restriction Digest Laboratory

Agenda 11/9/16 Exam Unit 3 Intro to genetic engineering

Forensic DNA Analysis Introduction to DNA.

Share one activity you did over the break.

Do Now If you could clone (make a copy) of any LIVING thing – what would you clone? Why? If you could pick your baby’s characteristics, would you?

Restriction Enzymes and Plasmid Mapping

Restriction Digest Laboratory

Biology Bellringer Choose Lab group

Opening Activity: March 5, 2018

Lab 8: PTC Polymerase Chain Reaction Lab

February 5, 2016 Objective: To be able to explain and model how DNA replicates To describe how the structure of DNA relates to how it replicates Journal:

Opening Activity: March 10, 2017

Opening Activity: Sept. 12, 2017 Collect your name tag and notebook.

Biotechnology: Restriction Enzyme Analysis of DNA

Opening Activity: March 12, 2018

1.Collect your planaria from your class drawer.

Opening Activity: May 8, 2017 Turn in worm explanation in basket!

Opening Activity: Feb. 27, 2017 Welcome Back! Share with your table how you experienced science during your break. Review your “Cell Cycle and Growth”

Restriction Digest Laboratory

Opening Activity: March 19, 2018

Simulating Genetic Screening

Cloning a DNA segment from bacteriophage

Opening Activity: March 20, 2017

Discussion Questions 1. Please describe the cell cycle in as much detail as possible. 2. Please describe DNA synthesis in as much detail 3.

Opening Activity: March 30, 2017

Electrophoresis.

Opening Activity: February 25, 2018

Bellwork: 1/11 Put your phone up  Week 1/7-1/11

30 Seconds 10 Time’s Up! 3 Minutes 1 Minute 4 Minutes Minutes

Presentation transcript:

Feb 3, 2015 I can… Predict how DNA is used to solve real- world problems Reminders: February 4 th – All first semester work must be turned in, please check the source! 1.DNA is a biomolecule, what atoms do you predict DNA is made from? 2.What happens during interphase of the cell cycle that prepares the cell for mitosis?

Feb 4, 2015 I can… Construct a DNA model and identify the 4 nucleotides that make up DNA. Reminders: All first semester work must be turned in TODAY! 1.Pick up reading guide at r. table – read, highlight, underline, etc. A.What does DNA stand for? B.What is the name of the sugar molecule in the DNA molecule? C. What is complimentary base pairing? D. How are genes related to DNA?

Feb 5, 2015 I can… Identify steps in DNA replication to ensure an exact copy is prepared for the daughter cells.. Reminders: All first semester work must be turned in TODAY – LAST CHANCE!!!! Quiz – Fri 2/13 (DNA, Replication & Pipets) 1. Suppose you know that the sequence on one strand of DNA is AGCTCAG. What is the sequence of the bases on the opposite strand? 2.What are the subunits of DNA? 3.Missing a 12 th night book? Black fleece?

Feb 6, 2015 I can… Use a micropipet, accurately measure out small amounts of liquid. Reminders: Quiz – Fri 2/13 (DNA, Replication & Pipets) 1.Pick up micropipet notes at resource table tape in. I will stamp your coloring and questions. 2.Assume a 100-base pair DNA molecule contains 45 cytosine. How many adenines are there? 2.What molecule helps “unzip” DNA for replication? 3.Where do you think DNA replication takes place for a bacteria cell?

Feb 9, 2015 I can… Measure amounts or liquid accurately with a micropipet and use a centrifuge. Reminders: Quiz – Fri 2/13 (DNA, Replication & Pipets) HW Reading Questions Opening Activity: What is a micropipette? Which pipette would pick up: 8 µl, 32 µl, 155 µl? 1 ml = __________ µl

Feb 10, 2015 I can… Explain how DNA can be manipulated by restriction enzymes. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! 1.I will stamp reading “Landmark Discovery…” 2.What do we mean by a balanced centrifuge? 3.What are the steps to release liquid from your pipette? 4.What is a restriction enzyme?

Feb 11, 2015 I can… Create a recombinant DNA. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! Opening Activity: 1.I will stamp restriction enzyme practice. 2.How do restriction enzymes help with manipulating DNA? How do they work? 3. If a strand of DNA was recognized by EcoRI 3 times, what would the final DNA look like?

Feb 12, 2015 I can… Explain the evolution of DNA manipulation. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! Opening Activity: 1.Pick up reading at resource table, Manipulating DNA, tape in. 2.Define the following: plasmid, restriction site, recombinant DNA.

Feb 13, 2015 I can… Share my knowledge of DNA, DNA Replication and micropipettes. Explain gel electrophoresis Homework: Manipulating Genetic Material stamped 2/18 Look at class website or Sample NB to stay caught up! Opening Activity: 1.Have your DNA vocab sheet available, Ms. Erb will come around and stamp. 2.Be sure to check class website, sample notebook, table of contents and the file folders if you were gone. Please be caught up when you return on Wednesday.

Feb 18, 2015 I can… Explain gel electrophoresis and demonstrate a model sample “run” of DNA. Homework: Biotech QUEST 3/4 Welcome back! 1.I will stamp your “DNA Goes to the Races Reading”, if you did not finish last week, finish now. 2.What is a gel electrophoresis?

Feb 19, 2015 I can… Load a gel to study DNA. Perform a practice digest with elephant DNA. Homework: Biotech Test March 4th Elephant DNA Practice Opening Activity: 1.Find “Practice Gel Formation and Loading of Gel”, READ. Set up your Gel box so you are prepared to pour a gel. 2.How much agarose will you measure out and pour into the gel box? 3.How long will it take for the gel to “set”?

Feb 20, 2015 I can… Prepare DNA to study on a gel. Digest the ivory DNA with restriction enzymes. Homework: Biotech Test March 4th 1.I will stamp your Elephant DNA practice. 2.Pick up Lab #17, tape in and READ. 3.What solutions will you put into your tube to perform the digest of the ivory DNA? 4.When you are measuring out 3µl, which pipet will you use?

Feb. 23, 2015 I can… Load a gel with DNA to study the banding patterns from elephants and discover where origin of the ivory. Homework: Biotech TEST 3/4 Opening Activity: 1.Read Part #2 from Elephant Lab in journal… a.What will we use today to cover the gel, instead of water? b.What will you add to each tube today? c.At what voltage will our DNA run today?

Feb 24, 2015 I can… Explain polymorphisms and how it causes different fragment sizes of DNA. Describe where E. DNA was collected. Homework: Biotech TEST 3/4 Opening Activity: 1.Pick up two items at Resource Table to tape into journal: Map of Africa Elephant Video Questions

Feb. 25, 2015 I can… Interpret elephant DNA banding patterns from our gels and determine where origin of the ivory. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report 2/27 Opening Activity: 1.Pick up the following items at the resource table a.Biotechnology Review Sheet – TAPE IN b.Orange/Yellow Data Submission Form 2.What is a banding pattern and how will we use them to determine the origin of our confiscated ivory?

Feb 26, 2015 I can… Interpret elephant DNA banding patterns from our gels and determine where origin of the ivory. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report 2/27 Opening Activity: 1.Pick up Lab #17 Elephant DNA Rubric - READ 2.What will be included in your claim for your lab report? 3.What is the scientific explanation for why two elephants different banding patterns? Why would two elephants have the same banding patterns?

March 2, 2015 I can… Explain sustainability and practice application EOC questions. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report Tues Opening Activity: 1.Turn in Lab #17 report to basket, if completed. Are all the lanes labeled? 2.Pick up Sustainability and Application packet at resource table.

March 3, 2015 I can… practice application EOC questions. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report Today 1.Turn in Lab #17 report to basket, due today! Are all the lanes labeled? 2.Find your EOC Practice Handout. I will stamp practice problem #1. 3.Continue working on ALL the practice problems, INDIVIDUALLY!