MIPS Molecular Imaging Program at Stanford School of Medicine Department of Radiology Stem Cell Therapy & Cardiovascular Molecular Imaging Joseph Wu, MD, PhD Department of Medicine/Cardiology Department of Radiology/Nuclear Medicine Stanford

Coronary heart disease is the #1 cause of morbidity and mortality in the US. CHF is the #1 cause of hospitalization for those age >65 yo. Annual health care costs related to cardiovascular diseases was ~ $220 billion last year. Stem cell transplant is a promising and exciting therapy. Background 2004 American Heart Association Update Orlic D, et al. Nature 2001

Bone marrow stem cells (BMSC) Endothelial progenitor cells (EPC) Mesenchymal stem cells (MSC) Skeletal myoblasts (SKM) Embryonic stem cells (ESC) Cardiac stem cells (CSC) Cardiac progenitor cells (isl1+) Available Stem Cells **Stem cells are capable of self-renewal, transformation into dedicated progenitor cells, and differentiation into specialized progeny

Wu JC et al, Circulation 2003 Imaging Embryonic Cardiomyoblasts Survival Optical ImagingMicroPET Imaging

Imaging Bone Marrow Stem Cells Engraftment in Living Subjects Ahmad Sheikh

horizontalcoronalsagittal [18F]-FHBG [18F]-FDG Fusion 0%ID/g 1%ID/g 0%ID/g 3%ID/g PET Imaging of ESC Transplant

PET Imaging of Teratoma Formation and Selective Ablation by GCV Treatment Feng Cao

Imaging ES Cell Differentiation into Cardiomyocytes Nkx 2.5-eGFP 12 days after embryoid body differentiation

Comparison of BMMNC vs MSC vs HSC Imaging of stem cell migration & homing Tissue engineering based on ESC EPC and diabetic cardiomyopathy Isolation of resident cardiac stem cells SiRNA knockdown of gene expression Other Projects