Welcome to the Media Cybernetics 2003 Dealer Conference Silver Spring, San Diego, Munich and Singapore August/September 2003

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Day One –9:00AM Welcome & Opening Remarks –10:00AM-Noon Image Pro Plus Five-0 –Noon-1:00PM Lunch –1:00-2:00PM Image Pro Plus Five-0 (Cont.) –2:00-3:00PM SharpStack Five-0 –3:00-4:00PM 3D Constructor Five-0 –4:00-5:00PM Scope-Pro Five-0 Day Two –9:00-11:00AM What is IQbase? –11:00-Noon How Can WE Demonstrate & Sell IQbase? –Noon-1:00PM Lunch –1:00-2:00PM Business Meeting –2:00PM Adjourn Agenda for 2-Day Program

Image-Pro Plus 5.0 More Answers on your Images! Image-Pro Plus Five-O

Image-Pro Plus 5.0 Some Highlights

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Alignment Correction Automatic Tiling and Stitching Tracking –Automatic and manual –Measurement Display Options Exciting New Features

Confidential - Do not copy or distribute Improved Stack Format Support –Handle stacks better and automatically assign colors where applicable. Color Composite Improvements –Customize Dyes and Colors –Image Set and Existing Image Knowledge New Basic Capture Dialog Introducing Image-Pro Plus 5.0! Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! New Processing and Display Tools –Despeckle Filter –L*A*B Color – Scale Bar –EDF Test Strips –Local Zoom –Synchronized Playback of Sequences –Synchronized Pan and Zoom –Surface Plot Overlay from EDF Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Snap to New Image –Allows users to automatically burn measurement overlays onto an image Set Manager –Multidimensional viewer - includes aspects of the improved sequence file support Spatial Calibration Improvements –List Management –System Calibration –Wizard Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Workspace Context Menu Additions –Local Zoom, dye tints, set capture AOI appear in context menu Version Information –Helps tech support know which versions of Image- Pro Plus and any plug-ins are running Default Settings Folder –Location where all settings and environment information is stored for the product. Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Objective Information File –Important for those that work with microscopes; links to Scope-Pro and AFA Display Range/Contrast Enhancement Interaction –User interface enhancement to make these tools less independent Dye Management –Allows for custom dyes and for user to define color by emission wavelength –Pre-Defined Dyes –Add New Dyes –Tint Commands Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! New Graphical User Interface Toolbar Changes –Toolbar has added functionality New Menu Structure –Menu is more logical for new users. Exciting New Features

Confidential - Do not copy or distribute Introducing Image-Pro Plus 5.0! Very New! WorkFlow Manager –User defined function –Toolbar oriented Very New! User Identities –Login –Basic, Full, Biological, Industrial defaults Exciting New Features

Image-Pro Plus 5.0 Key Selling points Hands On

Confidential - Do not copy or distribute IPP 5.0 Alignment Correction Align images or a sequence in translation, rotation, and/or scaling. Potential applications would be alignment of: –images taken over time with movement –channels of multiple images from a beam splitter –Serial sections –correcting for offset angle of a Greenough (off- axis) stereo microscope

Confidential - Do not copy or distribute IPP 5.0 Alignment Correction

Confidential - Do not copy or distribute IPP 5.0 Alignment Correction

Confidential - Do not copy or distribute IPP 5.0 Alignment Correction

Confidential - Do not copy or distribute Synchronized Playback of Sequences Synchronized Pan and Zoom Other Tools New Tools from the Solutions Zone

Confidential - Do not copy or distribute EDF Test Strips and EDF Surface Plot EDF Tools New Tools from the Solutions Zone

Confidential - Do not copy or distribute EDF Tools New Tools from the Solutions Zone

Confidential - Do not copy or distribute Automatic Tiling and Stitching Align and stitch images together using a Fourier transform edge detection algorithm based upon both pattern and contrast matching Options provide for the insertion of blank images, image order modification, and manual adjustment of pane location This will be useful for constructing panoramic views or for linking together multiple images taken with a motorized or non-motorized stage.

Confidential - Do not copy or distribute Automatic Tiling and Stitching

Confidential - Do not copy or distribute Automatic Tiling and Stitching

Confidential - Do not copy or distribute Automatic Tiling and Stitching

Confidential - Do not copy or distribute Tracking Manual Tracking –Click on objects frame by frame Automatic Tracking –Highlight objects of interest and let Image-Pro do the heavy lifting Graphs generated automatically –Cumulative and point to point options Many Measurement Options –Distance, velocity, etc.

Confidential - Do not copy or distribute Tracking

Confidential - Do not copy or distribute Tracking

Confidential - Do not copy or distribute NEW Acquire Dialog! New Basic Capture Dialog –Improved Nomenclature –Vertically aligned to conserve real estate –Most-used controls in one basic dialog *AND* –AutoSet button – a one-button solution which: Resets camera Performs Auto-White Balance (color only) Calculates Auto-Exposure Opens preview pane.

Confidential - Do not copy or distribute NEW Acquire Dialog! Quickly assign and change settings Lock Preview and Acquire Settings AutoSet!AutoSet!

Confidential - Do not copy or distribute NEW Acquire Dialog!

Confidential - Do not copy or distribute Spatial Calibration Improvements System Calibration applied when a new image is captured in Image-Pro, Scope-Pro, or AFA New Calibration Wizard Automatic correction for binning in Scope-Pro

Confidential - Do not copy or distribute Spatial Calibration Improvements

Confidential - Do not copy or distribute Spatial Calibration Improvements

Confidential - Do not copy or distribute New Tools from the Solutions Zone Color and orientation are easily chosen Scale Bar

Confidential - Do not copy or distribute Local Zoom New Tools from the Solutions Zone

Confidential - Do not copy or distribute Measurement Overlay Allows users to save measurement overlays burned onto an image Press to snap measurements to image

Confidential - Do not copy or distribute Workspace Context Menu Additions - Right-click features Local Zoom Dye Tints Dye Management –Allows for custom dyes and for user to define color by emission wavelength –Pre-Defined Dyes –Add New Dyes –Tint Commands

Confidential - Do not copy or distribute Color Composite Improvements –Customize Dyes and Colors –Preset Dye List –Links to Set and Image Information

Confidential - Do not copy or distribute Color Composite Improvements Press to create new dye

Confidential - Do not copy or distribute Color Composite Improvements Select color by visual inspection or emission wavelength!

Confidential - Do not copy or distribute Settings Improvements Default Settings Folder –All settings and environment information is stored in this location Lens Information File –Linked to acquisition in Scope-Pro and AFA

Confidential - Do not copy or distribute New Workflow Manager User-Defined Workflow Toolbar –Use Existing Menu Icons –Create New Icons –Include Self-Contained Macros without External Parameters

Confidential - Do not copy or distribute New Workflow Manager

Sharp Stack 5.0

Confidential - Do not copy or distribute Sharp Stack 5.0 Improved Speed of calculations Spherical Aberration correction Included Algorithms –No Neighbor –Nearest Neighbor –Inverse Filtering

Confidential - Do not copy or distribute Sharp Stack 5.0

Confidential - Do not copy or distribute Sharp Stack 5.0

Confidential - Do not copy or distribute Supplementary AutoQuant Modules SharpStack meets the majority market needs AutoDeblur Gold WF (widefield fluorescence) –Contains blind (2D & 3D) deconvolution methods AutoDeblur Gold CF (confocal fluorescence) –Contains confocal deconvolution methods AutoDeblur Gold WCF (all of the above) AutoDeblur 2D (only single plane blind deconvolution)

3D-Constructor 5.0

Confidential - Do not copy or distribute 3D-Constructor 5.0 More Visualization effects Manual Measurements Automatic Measurements –3D Count/Size –Histogram object selection 4D Measurements More Macros

Confidential - Do not copy or distribute 3D-Pro Series Competition Very high prices Small companies with mixed support capability Mixed distribution strategy Poor dealer relationships Poor integration into mainstream 2D image analysis packages

Confidential - Do not copy or distribute 3D-Pro Series 3D Constructor: Volume Rendering

Confidential - Do not copy or distribute 3D-Constructor Manual Measurements

Confidential - Do not copy or distribute 3D-Constructor Automatic Volumetric Measurements

Confidential - Do not copy or distribute 3D-Constructor Automatic Volumetric Measurements Over Time 4D Tracking of objects!!

ScopePro 5.0

Confidential - Do not copy or distribute Scope-Pro 5.0 Improvements Improved minimal dialogs to save real estate Acquire up to 999 Z-stack images X-Y stage initialization retained between sessions Calibration Improvements –Light path and magnification changers modify calibrations Site map for user defined capture regions

Confidential - Do not copy or distribute Scope-Pro 5.0

Confidential - Do not copy or distribute Scope-Pro 5.0

AFA 4.5 Advanced Fluorescence Acquisition

Confidential - Do not copy or distribute Advanced Fluorescence Acquisition (AFA) For customers who acquire complex image sets Multiple color channels (usually fluorescence) or microscope modes Time-lapse, motion studies Stage position –Multiwell plates –Area scans –User defined locations Multiple focal planes (Z-stack)

Confidential - Do not copy or distribute Advanced Fluorescence Acquisition (AFA) Enhanced Capabilities Time-lapse Multi-channelMultiwell plates Z-stackScan area User-defined Stage Positions

Confidential - Do not copy or distribute Advanced Fluorescence Acquisition (AFA) Interface: First Use

Confidential - Do not copy or distribute Advanced Fluorescence Acquisition (AFA) Interface: Preview Tab

Confidential - Do not copy or distribute Scope-Pro Device Updates Check our website for device updates

Camera Kit Update

Confidential - Do not copy or distribute Camera Kit Products CoolSNAPcf Evolution LC Evolution VF (NEW!) Evolution MP Evolution QEi

Confidential - Do not copy or distribute CoolSNAP-Pro cf Kits 1392 X 1040 pixels 12-bit chip, Cooled 5C below ambient Available in Bayer color or monochrome or with CRI Micro*Color Faster readout speed (20Mhz.) Preview at frames/sec. - full resolution mono Significantly Improved Sensitivity! Reduced dark current noise down to <2 electrons! Supports longer integration for fluorescence applications Camera List - $4,999 Dealer Discounts up to 36%!

Confidential - Do not copy or distribute Evolution LC™ Integrated Camera Kit Digital alternative to video microscopy Available in monochrome and color Features –Fully integrated with your choice of Image-Pro software –FireWire (IEEE 1394) C-mount camera –1.3 megapixel array –Up to 14 fps at 1280 x 1024 res., 30 fps at 640 x 480 –Laptop Kit

Confidential - Do not copy or distribute Evolution VF - cooled & uncooled version - mono & color Features –Designed for high resolution brightfield scientific and industrial applications. –A progressive scan interline CCD sensor gives a resolution of 1.4 million pixels in a 12-bit digital output. –High-speed low noise electronics provide linear digital data –Frame rates of up to 110 fps in region of interest and binning. –The IEEE 1394 FireWire™ digital interface allows ease of use

Confidential - Do not copy or distribute Evolution VF - Specifications CCD Sensor Light Sensitive Pixels1.4 million, 1392 x 1040 Binning Modes2x2, 4x4, 8x8 ROI (Region Of Interest)From 1x1 pixels up to full resolution, continuously variable Exposure/Integration Control 12µs to 17.9min. in1µs increments Sensor TypeSony ICX205 Progressive Scan Interline CCD, Monochrome, or Color Pixel Size4.65µm x 4.65µm Linear Full Well10,000 e - Read Noise12 e - Cooling AvailableYes Cooling TypePeltier Thermoel. cooling 25 degrees Celsius below ambient Digital Output12-bit Readout Frequency20, 10, 5, 2.5MHz Frame Rate10fps full 12-bits, 165fps maximum with binning and ROI

Confidential - Do not copy or distribute Evolution MP™ Integrated Color Camera Kit High resolution 5.5 megapixel color camera Features –Fully integrated with your choice of Image-Pro software –FireWire (IEEE 1394) C-mount camera –3-5 fps at 2560x1920(30-bit); 25 fps at 640 x 480 –Option for cooling –No interpolation or Pixel shifting

Confidential - Do not copy or distribute Evolution MP™ Integrated Color Camera Kit Imaging Chip Type 2/3” Sony ICX to 1 interlaced CCD color Resolution2588 x 1960 Pixel Size3.4µm x 3.4µm Readout Noise9.8 electrons Maximum Frame Rate3-5 fps at 2560 x 1920 (36-bit) ShutterElectronic shutter, no moving parts Cooling Type Optional Peltier thermoelectric cooling to 10°C below ambient Physical Weight710g Lens mount C-mount, 2/3” optical format for microscope or camera lens, plus a 1/4”-20 tripod mount Interface Operating SystemWindows® 98 SE, ME, 2000, XP Data Format10-bit Interface TypeIEEE 1394 FireWire Maximum Data Rate 20 MHz at 8-bit and 10 MHz at 10- bit

Confidential - Do not copy or distribute Evolution QEi™ Integrated Camera Kit High Quantum Efficiency camera Features –Fully integrated with your choice of Image-Pro software –FireWire (IEEE 1394) C-mount camera –5 fps at 1360x1036(12-bit)10 fps at 1360x1036(8-bit)

Confidential - Do not copy or distribute Evolution QEi™ Integrated Camera Kit Imaging Chip Type 2/3” Sony ICX-285 progressive scan interline CCD monochrome Resolution1360 x 1036 Light Sensitive Pixels Pixel Size6.45µm x 6.45µm Readout Noise8 electrons Dark Current0.15 electrons/pixel/sec cooled Maximum Frame Rate 10 fps full resolution in 8-bit, 5fps in 12-bit. Higher speed with binning and ROI functions (up to 4x4 binning) ShutterElectronic shutter, no moving parts Cooling Type Peltier thermoelectric cooling to 25°C below ambient Physical Lens mountC-mount, 2/3” optical format Interface Operating SystemWindows® 98 SE, ME, 2000, XP Data Format12-bit Interface TypeIEEE 1394 FireWire Maximum Data Rate10 MHz and 20 MHz selectable at 12-bit

IQbase Extracting Information From Images

Confidential - Do not copy or distribute Image-Pro IQbase Information and data management software for individuals and companies that need a flexible and powerful database solution

Confidential - Do not copy or distribute Image-Pro IQbase Object-Oriented Database –More powerful and faster searches than relational databases found in most competitive products –Compatible with SQL, Oracle, and other databases –Can be fully integrated with the world’s leading scientific image analysis software, Image-Pro Plus, which contains numerous input options, measurement and analysis tools, and world-class support

Confidential - Do not copy or distribute Image-Pro IQbase - Versatile Data can be stored in a variety of locations Data is organized according to the users needs and desires Saves Time

Confidential - Do not copy or distribute Image-Pro IQbase - Versatile Can work with MS-SQL, Oracle and other database types Data can be made available to web servers for customers/collaborators to view over the world wide web

Confidential - Do not copy or distribute Image-Pro IQbase - Expandable Suitable for single-user, small workgroup, and enterprise-level deployment Seats are easily added Database can handle virtually unlimited entries

Confidential - Do not copy or distribute Image-Pro IQbase - Intelligent Automatically remembers user settings and preferences Understands and collects data from within Image-Pro Plus automatically Reports can be produced in a variety of formats (PDF, HTML, RTF, etc.)

Confidential - Do not copy or distribute Image-Pro IQbase - Programmable Includes Visual Basic for Applications ® Create custom forms, tools and plug- ins from within the product

Confidential - Do not copy or distribute IQbase Top Features 1.Context Sensitive Searches 2.Easy Addition of files to the database 3.Image-Pro measurements savable and searchable 4.Savable searches 5.Multi-user login with optional passwords 6.Administration 7.Data display 8.Report generator produces a variety of formats 9.Customizable interface features 10.Visual Basic for Applications 11.Offline Storage

Confidential - Do not copy or distribute Who Will Buy IQbase? New Image-Pro customers (plug-in) –Archive measurements automatically Existing Image-Pro users –Network installations of IPP Near-line storage systems Researchers and organizations that need to improve their efficiency

Confidential - Do not copy or distribute IQbase Database product that includes programming, reporting, and image analysis features –Built for organizations that want to archive their data and mine it for information at a later time –Perfect for organizations that need to communicate their findings both internally and externally –Ideal for those interested in combining powerful data management with image analysis

Future Product Discussions

Business Session

Confidential - Do not copy or distribute HEADQUARTERS Media Cybernetics, Inc Georgia Avenue, Suite 200 Silver Spring, MD USA Phone: Fax: Web: