Berry Full of DNA: Exploring Properties of Strawberry DNA Honors Coordinated Science II Wheatley-Heckman

Background Every living thing contains cells, and these cells contain DNA. Using the right procedures, we can extract DNA from the cells of almost any living thing. Examples: The Human Genome Project, Genetic Testing, Crime Scene Investigations… http://learn.genetics.utah.edu/content/labs/extraction/

Why Strawberries? While human cells contain 2 sets of DNA…strawberry cells contain 8!! That is A LOT of DNA, and it is easy to see when extracted. Strawberries are easy to mash, and ripe strawberries produce enzymes that contribute to the breakdown of cell walls.

Procedure Overview First, you will break the cells apart by physically crushing the strawberries in a bag.

Procedure Overview You will use an extraction buffer (detergent solution) to dissolve the membranes of the cell. Then, you will filter the dissolved cells using a funnel and cheesecloth to remove unwanted particles. Ethanol is used to observe the DNA.

Important Notes Before beginning the lab, answer all the pre-lab questions. Read the procedure carefully before starting the lab. Wear safety goggles! Each table should work as 2 groups. When finished with the lab, answer all the analysis questions.

What did we see?