PERKIN-ELMER MODEL 200 UV-VIS SPECTROPHOTOMETER OPERATING INSTRUCTIONS

INSTRUMENT WARM UP Turn the power switch on. Turn on the tungsten lamp (W lamp). The tungsten lamp is used up to 370 nm. Allow the lamp to warm up for a few minutes. (30 minutes are necessary for stable operation). Set the source selector to the Visible lamp (out). Set the slit to the minimum, 0.2 nm. This setting gives sufficient resolution for recording spectra of gas-phase samples. Set the response to MEDIUM for now.

%T and Absorbance Calibration Depress the %T button. Insert the 0%T shutter (large plate) along the side of the sample compartment, blocking the sample beam.

%T and Absorbance Calibration Set on the digital display using the 0%T control. Turn control enough to see –00.0 then turn back until just appears. Remove the 0%T shutter. Set with the 100%T/ ABS. Control.

Absorbance Spectrum Push the 0-2 Abs button to switch to Absorbance readout. Set spectrophotometer SCAN SPEED selector to 30 nm/min. The drive gears should be arranged as shown below.

Absorbance Spectrum Set the RESPONSE switch to FAST, to follow the sharp gas-phase peaks. Place the sample cell in the sample compartment.

Absorbance Spectrum Manually set the initial, longest wavelength, in the monochromator. When setting wavelengths, always turn the WAVELENGTH control from longer to shorter wavelengths to avoid backlash in the gears for best reproducibility. Click the Start Green button on LoggerPro Switch the Scan Box switch to ON After data collection click the red Stop button in Logger Pro and turn off the Scan Box switch