Effect of Certain Agonists & Antagonists on Isolated Guinea-pig Ileum

Slides:

Advertisements

Similar presentations

SYNAPSES AND DRUGS.

Advertisements

How the Brain Works 1. Background: The Synapse 2.

Gut Motility By Dr. Aijaz. cross section of the intestinal wall layers from outer surface inward: (1) the serosa, (2) a longitudinal muscle layer, (3)

351 PHL 5th lecture Effect of Certain Agonist and Their Antagonist on Isolated Guinea Pig Ileum Awatif B. Al-Backer.

Awatif B. Al-Backer. Stimulants & their Blockers 1-Drugs acting on Nn R Agonist -Dil. Nicotine (0.01%, 0.1ml) -MOA: Dil. Nicotine stimulates Gs protein.

Awatif B. Al-Backer Function of the nerve innervations in the heart (Sympathetic or Parasympathetic): Alteration of the basic rhythm of the heart.

Awatif B. Al-Backer. Intestine  Involuntary structure with myogenic contraction (spontaneous rhythmic contraction) which undergo control of nervous system.

Neurotransmission B.Sc Archetypal neurotransmitter is acetylcholine; We know more about cholinergic transmission than any other. However, cholinergic.

HISTAMINE Histamine is formed from the amino acid histidine and is stored in high concentrations in mast cells. Localisation  lungs, skin, GIT Excess.

PHARMACOLOGISTS’ PERSPECTIVE ON COLON PHYSIOLOGY.

Serotonin & Anti-serotonin drugs At the end of this session students should be able to: Describe serotonin (5HT) receptors including serotonin & antiserotonin.

Neurotransmitters Many Neurotransmitters (NT) exist: -Dopamine -Adrenaline -Serotonin -Acetylcholine Drugs can either: –Increase the effect of certain.

Awatif B. Al-Backer. Purpose of Pharmacological Experiments 1. Qualitative: For determination of the activity or the mechanism of action of the drug,

313 PHL Lab # 2 Effects and Sites of Action of Different Agonists Drugs (Stimulant or Relaxant)on The Isolated Rabbit Intestine.

Dose response curve of Ach & the effect of different drugs on the isolated frog rectus abdominis Lab # 5.

Bioassay of histamine using guinea-pig ileum

Effect of Certain Agonists & Their Antagonists on The Isolated Rabbit Intestine Lab #3.

Pharmacology-1 PHL 313 Parasympathetic Nervous System Second Lecture By Abdelkader Ashour, Ph.D. Phone:

Widely Found and Studied Neurotransmitters  Glutamate – has excitatory functions  4 Ascending activating systems are very important  Cholinergic System.

Autonomic Nervous System Dr Dileep Verma Associate Professor Deptt of Physiology KGMU.

313PHL Lab #4 Identification of effect and site of action of an unknown drug using isolated guinea-pig ileum.

Pharmacology-1 PHL 313 Fourth Lecture By Abdelkader Ashour, Ph.D. Phone:

Effects of different drugs on isolated intestine.

Effect & Sites of Action of Different Agonist Drugs on The Isolated Rabbit Intestine Lab # 1.

Effect of autonomic drugs on rabbit’s isolated intestine

 Receptors are mostly membrane-bound proteins that selectively bind small molecules called ligands which results in physiological response.  They are.

Neurotransmitters & Receptors Lecture 10. Ligands & Receptors n Ligand l Neurotransmitters (NT) & Drugs n Receptor proteins l Control ion channels n NT.

Clinical Pharmacology Autonomic pharmacology Jane M Johnston Ph.D.

Chapter 11 Peripheral Nervous System. Peripheral Nervous SystemOutline Introductory Comments 1. Autonomic Nervous System  Parasympathetic versus sympathetic.

Synaptic Transmission Syllabus Toole page

Course contents Lab1 : Introduction to Pharmacology and Concept of ( Dose - Response Curve ). Lab2 : Effect of cholinergic Drugs on intestine and % of.

Serotonin ( 5- hydroxy tryptamine; 5HT ). Serotonin ( 5- hydroxy tryptamine; 5HT ) Locations: Locations: - Gut enterochromaffin cells ( 90% ) - Gut enterochromaffin.

Drugs that affect neuronal functioning by altering neuronal transmission in ways other than attaching to synaptic receptors.

NEURONS & NEURAL TRANSMISSION NEUROCHEMICAL MECHANISMS OF DRUG ACTION

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings The Synapse Key Stage 5 Biology.

CHOLINERGIC DRUGS Define cholinergic Drugs ?

Neurotransmitters: Monoamines

Neurotransmitters Information in this presentation taken from UCCP Content.

Effect of Atropine on the Smooth Muscles of the Airway

313 PHL Lab # 6 Effect & site of action of different drugs on isolated rabbit's heart. (Langendorff preparation)

PHC 221 Part(I) Dr. Huda Al Salem Lecture (5). Factors that affect the efficacy 1- Mechanism of biological response 2- Concentration-Response Curves.

Quiz, quiz, trade 1.Look back at the synapse and transmission of a nerve impulse sequence. 2.Write a question you can answer on a piece of card (put the.

Membrane Function Signal Transduction. I. Introduction to Receptors & Signal Transduction.

At the end of this lecture, the students will be able to: 1.Define receptors 2.Define agonists 3.List types of agonists 4. Define antagonists 5. List.

Nervous system Ⅱ Wei yuanyuan. Neurotransmitter  Definition : a small molecules released by presynaptic neuron into synaptic cleft that cause a change.

1 PHL. 313 Dr. Khairy Zoheir. Lab.1 INTRODUCTION TO NS PHARMACOLOGY and DOSE- RESPONSE CURVE.

INTRODUCTION TO AUTONOMIC PHARMACOLOGY: Part VI Interaction of drugs with the autonomic nervous system.

parasympathetic agents

313 PHL Lab # 1 Effects and Sites of Action of Different Agonists Drugs (Stimulant or Relaxant)on The Isolated Rabbit Intestine.

Acetylcholine & Other Neurotransmitters

Department of Pharmacology

CHOLINERGIC TRANSMISSION

DRUG ANTAGONISM DR. SHABANA ALI.

Triple response of histamine

313 PHL Lab # 2 Effects and Sites of Action of Different Agonists Drugs (Stimulant or Relaxant) on The Isolated Rabbit Intestine.

313 PHL Lab # 3 Effect of Certain Agonists & Their Antagonists on The Isolated Rabbit Intestine.

Dr.Muhammad Ahmed. Contents Ans Sympathetic Parasypathetic Muscaranic receptors.

Neurotransmitters (NT)

IB Psych 10/10/17 Today’s Agenda:

SYNAPSES AND DRUGS.

SYNAPSES AND DRUGS.

ADRINERGIC DRUGS By Dr. Mirza Shahed Baig.

Adrenergic antagonists

Chapter 2 Neural Communication.

SYNAPSES AND DRUGS.

Module 1 Communication and homeostasis

ANTAGONISTS Antagonists are substances which block the action of agonists There are a number of types COMPETITIVE NON-COMPETITIVE PARTIAL AGONISTS PHYSIOLOGICAL.

Biological standardization and screening of drugs

Presentation transcript:

Effect of Certain Agonists & Antagonists on Isolated Guinea-pig Ileum Lab #4

Objectives Discuss the types of receptor present in guinea-pig ileum Explain the action of agonists & their specific antagonists Discuss the reasons for using guinea-pig ileum Explain of effect of different drugs on isolated guinea-pig ileum curve

Types of receptors in GPI 1- Cholinergic receptors 2- Serotonergic or tryptaminergic receptors (5-HT1→5-HT7) 3- Histaminergic receptors

Cholinergic receptors 1- NN R Agonist: Dil. Nicotine Antagonist: Conc. Nicotine 2- M3 R Agonist: Ach Antagonist: Atropine

Serotonergic or tryptaminergic receptors Serotonin = 5-HT(5-hydroxytryptamine) 5-HT receptors in GPI: 5-HT2A Agonist: serotonin Antagonist: ketanserin methysergide cyproheptadine (blocks 5-HT2A & H1)

Histaminergic receptors R subtype Distribution Action Agonist Antagonist H1 Smooth muscles contraction Histamine Mepyramine cyproheptadine H2 Gastric mucosa Gastric acid secretion Cimetidine Ranitidine H3 Presynaptic Autoregulation of H release Thioperamide N.B. Histamine acts by stimulating Gs protein → ↑ cAMP → ↑ [Calcium]ic → contraction

e.g. Barium chloride e.g. Papaverine Direct acting drugs Direct spasmogen e.g. Barium chloride Direct spasmolytic e.g. Papaverine

Advantages of GPI No myogenic contraction More sensitive to histamine

Experiment N Key: Hist.=histamine AH= antihistamine 5-HT=serotonin W N 0.1ml Bacl2 0.1ml 5-HT Ach 0.1ml Hist. 0.1ml W+N W+N Ach 0.1ml AH 0.2 ml Hist. 0.1ml Bacl2 0.1ml Atropine 0.1ml 5-HT 0.1ml Papaverine 0.1ml Key: Hist.=histamine AH= antihistamine 5-HT=serotonin

Experiment Identification of site of action of an unknown drug using isolated guinea-pig ileum N W N W+N W+N Ach 0.1ml Hist. 0.1ml Unk. Unk. Ach 0.1ml Hist. 0.1ml Atropine 0.1ml AH 0.2 ml Key: Ach= acetylcholine Hist.=histamine AH= antihistamine Unk.= unknown