THE CITRIC ACID CYCLE Kadek Rachmawati

The citric acid cycle = Kreb’s cycle = Tricarboxy- lic acid cycle a series of reactions that oxidized Asetyl-CoA Occur in Mitochondria To yield energy (ATP) TCA cycle the final common pathway for the aerobic oxidation of carbohydrate, lipid and protein

TCA cycle is common pathway for carbohydrate, lipid and protein

TCA cycle pathway

The function of TCA cycle : 1. Oxidized of Acetyl CoA ATP ( 1 mol Acetyl CoA 12 mol ATP ) 2. The citric acid cycle is AMPHIBOLIC : - it can oxidized to yield ATP - it is important in the provision of carbon skeletons for gluconeogenesis, fatty acid synthesis and interconversion of amino acid

Vitamins play key roles of the Citric Acid Cycle Four of the B vitamins are essential in the citric acid cycle : 1. Ribovlavin FAD 2. Niacin NAD 3. Thiamin TPP 4. Pantothenic acid CoA The B vitamins are essential in energy yielding metabolism by dehydrogenase enzyme : - FAD 2 mol ATP - NAD 3 mol ATP

INHIBITOR OF TCA CYCLE 1. Fluoroacetate Condensation with CoA Fluoroacetyl-CoA Condensation FluoroacetylCoA with Oxaloace- tate Fluorocitrate inhibit Akoni- tase enzyme accumulation of citrate Fluoroacetate pesticide 2. Malonate suksinat dehydrogenase enzyme 3. Arsenite α-ketoglutarate dehydrogena- se enzyme

Participation of TCA cycle in fatty acid synthesis from glucose

Involvement of TCA cycle in transamination and gluconeogenesis

REGULATION OF TCA CYCLE

REGULATION OF TCA CYCLE

REGULATION OF TCA CYCLE

REGULATION OF TCA CYCLE

Thank You