The comparing of natural antibacterial substance on E. Coli Manhasset Science Research Jean Cheng Period 4

Need Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil

Knowledge Base Figure 2: Chemical formula for Penicillin mistry/wp- content/uploads/2008/04/peni cillin-core1.png

Knowledge Base (cont) Figure 3: Tea tree’s terpene chemical formula ges/R67_IV-1_ _8959.jpg Figure 4: Pectin Chemical formula

Figure 5: Aloe vera Knowledge Base (cont)

Figure 6:Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008) 96DFD197.gif Literature Review

Literature Review (cont) Figure 7: Antimicrobial activity of aloe vera gel (Alemdar, et al. 2009)

Literature Review (cont) Figure 8:Yuzu fruit seed oil on different microbial (Nakazono, et al. 2006)

Literature Review (cont) Figure 9:12 different essential oil on different species of bacteria. (Rusenova, et al. 2009)

Purpose Find which natural substance is best in inhibiting E. coli. Null Hypothesis: No effect Alternative Hypothesis: Tea tree oil is the best antimicrobial substance against E. coli

Methodology The comparing of natural antibacterial substance on E. Coli Essential oil and antibiotic plant substance N=12 Garlic (allicin) N=3 Tea Tree oil N=3 Petri dish, E.coli, filter paper, glass spreader Disc diffusion assay method SPSS ANOVA Test Aloe (anthraquinones) N=3 Yuzu fruit oil N=3 Millimeter

Protocols Bacteria on agar Inhibited Zone(mm) Paper disk with substance being tested Figure 10: Disk Diffusion method

Protocols (cont) Figure11: obook/images/fig10_5.jpghttp://gsbs.utmb.edu/micr obook/images/fig10_5.jpg

Do-Ability Not expensive doesn’t require large amount of space Some materials found in lab or supermarket Can be finished in 3 days E. coli is needed as well as agar, aloe, tea tree, yuzu, and garlic

Budget

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