RNA interference: Kill the messenger!. conclusion: dsRNA triggers potent and specific gene silencing inject worms with dsRNA corresponding to.

Slides:

Advertisements

Similar presentations

Dogmatic View of Gene Expression DNAProteinRNA Post-transcriptional Control: Quantitative Control: Levels of mRNA not proportional to levels of mRNA synthesized.

Advertisements

Rhiana Lau MMG C174 Professor Simpson

Reverse Genetics in Drosophila

Gene 2 Nerve cells Neuron-specific enhancer P2 T Ex2Ex3 Ex4I3I2 Cell-specific gene expression gene 1 P1 T Ex1Ex2 Ex3I2I1 Ex1 I1 Skin cell-specific enhancer.

RNA interference (RNAi)

Dicer-Like A RNA Helicase Gene Involved in Gene Silencing UCSF Max BachourJessica ChenChris McQuilkin.

Analyzing the effects of a gene-specific siRNA on IL13R-α2 mRNA and protein levels in glioblastoma multiforme cells INTRODUCTION  RNA interference (RNAi)

Post-Transcriptional Gene Silencing (PTGS) Also called RNA interference or RNAi Process results in down-regulation of a gene at the RNA level (i.e., after.

RNA Interference (RNAi) Pathway

Biochemistry April Lecture RNA Interference (RNAi) (see also siRNA, micRNA, stRNA, etc.)

Distinct Roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA Silencing Pathways Lee, S.Y., Nakahara, K., Phan, J.W., Kim, K., He, Z., Sontheimer,

Advantages of C. elegans: 1. rapid life cycle 2. hermaphrodite

Post-transcriptional gene silencing

Clicker question The 2006 Nobel Prize in Medicine was given to Andrew Fire and Craig Mello for their work showing that ____ can regulate the expression.

The History of RNAi John Doench Insight and discovery are functionally separable. The one precedes the other. Insight can happen every day. Discovery does.

Epigenetics Course Title: Lecture Titles:

Mammalian RNAi pathways Michael T. McManus MIT Center for Cancer Research.

RNAi. What is RNAi? RNA-based mechanisms of gene silencing. These siRNAs are bound by a protein-RNA complex called the RNA-induced silencing complex (RISC)

Post-Transcriptional Gene Silencing (PTGS) Also called RNA interference or RNAi Process results in down-regulation of a gene at the RNA level (i.e., after.

Molecular Cellular Biology-I (MCB-I) PCB 6025 M. Alejandro Barbieri M. Alejandro Barbieri Office: HLS 318C/214 Hours: by appointment.

RNAi Haixia Wang

Transfection. What is transfection? Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing.

BEH. 109: Laboratory Fundamentals in Biological Engineering

Regulatory RNAs. Cells produce several types of RNA.

RNA interference Definition: RNA interference (RNAi) is a mechanism where the presence of certain fragments.

MBP1007/ Nucleic Acids A functional mRNA: The cytoplasmic story Objectives (1) To discuss the iNUTS and iBOLTS of how mRNAs function in the cytoplasm.

SiRNA and Epigenetic Asma Siddique Saloom Aslam Syeda Zainab Ali.

RNAi Mechanism. The Central Dogma DNA (double-stranded) RNA (single-stranded) Protein.

Changes to Syllabus: Quizzes put back: Change Oct. 3 to Oct. 17

Carly Sproule BE  Mechanism that silences a gene so that the protein associated with it is not expressed  Regulates gene expression  Used as.

What is RNA interference?

Epigenetics: RNAi and Heterochromatin formation

RNAi Mitchell Planty. Quick Overview RNAi– Natural regulatory mechanism for gene expression. – Protection mechanism against overproduction of harmful.

Welcome Everyone. Self introduction Sun, Luguo ( 孙陆果） Contact me by Professor in School of Life Sciences & National Engineering.

Drosha. 121th Lab meeting 석사 2 년 박 은 실.

Arabidopsis thaliana Response to Tobacco Rattle Virus Jessica Martin, Cory Zoetewey, and Lisa K. Johansen, Department of Biology University of Colorado.

Gene regulation in biological responses Hypothesis testing Differentiation ProliferationApoptosis High throughput 96 well plates array based Single gene.

Conditional systems - principles Conditional systems may function on the basis of: - regulatory proteins - aptamers - allosteric ribozymes - antisense.

Caenorhabditis elegans

Biochemistry 412 RNA Interference (RNAi) (see also siRNA, micRNA, stRNA, etc.) 8 April 2005 Lecture.

The Power of “Genetics” LOSS OF FUNCTION Easy in yeast Difficult in mammals Powerful tool to address roles in developmental or signaling networks Gene.

Patenting Interfering RNA John LeGuyader – SPE Art Unit 1635 (571)

The mechanism of RNAi by Renchang. Guo S et al, a gene required for establishing polarity in C. elegans embryos, encoding a putative Ser/Thr kinase that.

RNA-ligand interactions and control of gene expression

Regulatory Relationship of microRNAs in Alzheimer and Huntington Diseases: A Cladistics Approach Prachi Srivastava AMITY Institute of Biotechnology AMITY.

Vectors for RNAi.

By: Jack Liao- HOSTOS- Lincoln Academy of Science Jingwei Zhang- H.S. for Dual Language and Asian Studies Gabriell Copeland- Eagle Academy For Young Men.

Atta ur Rahman school of biological Science National University of Sciences & Technology CMB-234:Molecular Biology.

Ch 16. Posttranscriptional Regulation RNA interference (RNAi)

An Introduction to RNA Interference

Conditional systems - principles

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

Figure 2 Dicer and RISC (RNA-induced silencing complex).

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

Mammalian RNAi pathways MIT Center for Cancer Research

Short interfering RNA siRNA

Short interfering RNA siRNA

Short interfering RNA siRNA

Knocking out the Argonautes

RNA interference: the new somatic cell genetics?

Antti Nykänen, Benjamin Haley, Phillip D. Zamore Cell

Introduction to C. elegans

RNAi: Insight, Mechanisms and Potential

Engineering parathyroid cells to treat secondary hyperparathyroidism

The Power of “Genetics”

Presentation transcript:

RNA interference: Kill the messenger!

conclusion: dsRNA triggers potent and specific gene silencing inject worms with dsRNA corresponding to a gene involved in wiggling (unc-22) Discovery

Biochemistry to Examine Structure short interfering RNA: siRNA 21 nt long, dsRNA: P P

Dicer generates siRNAs RNase III endonuclease Mix cell extract with dsRNA 21-mer Berstein et al. Nature (2001)

RNA interference (RNAi) dsRNA siRNA Dicer RISC AAAA cap mRNA AAAA cap mRNA

Timeline cosuppression of purple color in plants dsRNA injection in worms short RNAs identified in plants RNAi shown in vitro RISC activity partially purified siRNAs identifiedDicer identified RNAi used against HIV genome-wide RNAi screens begin

Big Potential for Small RNAs Adapted from Ryther et.al. Gene Therapy (2005)

S2 Drosophila cells spread on coated glass surface via lamellipodia

Example of RNAi experiment