Extraction of DNA From Wheat Germ (Your Name) Cell Biology Period 6 November 6, 2006

What is DNA? DNA is a nucleic acid called deoxyribonucleic acid. DNA is a nucleic acid called deoxyribonucleic acid. Its structure is a double helix Its structure is a double helix It contains all of an organisms hereditary information It contains all of an organisms hereditary information … …

What is Wheat Germ? Explain where wheat germ comes from Explain where wheat germ comes from Explain why it contains a large amount of DNA Explain why it contains a large amount of DNA What does it look like? What does it look like?

Extraction of DNA Experiment Supplies List A list of the supplies and equipment that you need to conduct your experiment to extract DNA from the raw wheat germ A list of the supplies and equipment that you need to conduct your experiment to extract DNA from the raw wheat germ Explanation of what each supply does in your protocol Explanation of what each supply does in your protocol

Supplies List Test Tube Test Tube Raw Wheat Germ Raw Wheat Germ Warm Water Warm Water Non-Iodized Salt Non-Iodized Salt Detergent (Dawn) Detergent (Dawn) Ice Cold Ethanol Ice Cold Ethanol Meat Tenderizer (Adolph’s) Meat Tenderizer (Adolph’s) Wooden Splint or Glass Stirring Rod Wooden Splint or Glass Stirring Rod Baking Soda Baking Soda

Extraction Protocol Step by step procedure that you will follow to extract the DNA from raw wheat germ Step by step procedure that you will follow to extract the DNA from raw wheat germ Make sure the font is big enough for viewers to see Make sure the font is big enough for viewers to see Use more than one page if your protocol is long Use more than one page if your protocol is long

Extraction Protocol 1. Weigh out 1.5 g of raw Wheat Germ and place in test tube 2. Add enough warm water to cover and dissolve the wheat germ 3. Add 5ml of liquid detergent to test tube 4. Add 3g of meat tenderizer to test tube 5. Add a small amount of baking soda solution to the test tube 6. Add 1g of salt to test tube

Extraction Protocol 1. Pour 10ml of ice cold ethanol into the test tube. 2. Let the test tube stand for a few minutes to allow the DNA to precipitate from solution. 3. DNA will form as a white, cottony substance in the solution. 4. Collect the DNA material by spooling it up with the wooden splint. 5. Let the DNA dry on a paper towel.

What are the Results of the DNA Extraction?

Thank You Page (Sources)