Production of Erythropoietin in Pichia Pastoris Eporis – Team 15 Team Background Problem Design Alternatives Design Norms
Team Eporis Abby Leistra Stephen Tubergen Zion Lee Nick Giles Team Background Problem Design Alternatives Design Norms 1/7
Background: What is Erythropoietin? Erythropoietin (EPO) is a glycoprotein hormone Promotes red blood cell production Naturally made in kidneys Treats anemia caused by Chronic kidney disease Cancer chemotherapy HIV therapy $13 Billion Market glycam.org Team Background Problem Design Alternatives Design Norms 2/7
Problem Expression: Chinese Hamster Ovary (CHO) Cells An eight-week treatment can cost up to $11000 Well studied Expensive feed Slow growth rate http://nikon2.magnet.fsu.edu Team Background Problem Design Alternatives Design Norms 3/7
Solution? Expression: Pichia Pastoris cells Relatively new Cheap feed (methanol) Fast growth rate 2013.igem.org Team Background Problem Design Alternatives Design Norms 4/7
Design Alternatives Pichia Pastoris Strain Erythropoietin Structure Turn on and off right genes Erythropoietin Structure Glycosylation and other modifications glycam.org Team Background Problem Design Alternatives Design Norms 5/7
Design Alternatives Bioreactors Separation Units Team Background Mianbin Wu, et. al. (2011). DOI: 10.5772/20246. Bioreactors Separation Units Team Background Problem Design Alternatives Design Norms 6/7
Design Norms Stewardship Transparency Trust Team Background Problem Design Alternatives Design Norms 7/7
Questions?