Tomáš Bílý, Martin Šemro 28. 07. 2011 Simultaneous AFM and light microscopy of live cells: nano- and microworld within one picture.

Slides:

Advertisements

Similar presentations

Scanning near-field optical microscopy (SNOM) for magneto-optics Paolo Vavassori INFM - National Research Center on nanoStructures and Biosystems at Surfaces.

Advertisements

AFM Basics Xinyong Chen.

The Principle of Microscopy : SEM, TEM, AFM

SCANNING PROBE MICROSCOPY By AJHARANI HANSDAH SR NO

Lecture 10. AFM.

AFM-Raman and Tip Enhanced Raman studies of modern nanostructures Pavel Dorozhkin, Alexey Shchekin, Victor Bykov NT-MDT Co., Build. 167, Zelenograd Moscow,

Spectroscopy is the study of interactions between light and matter. Photoinduced absorption spectroscopy can show us which materials (such as quantum dots)

Basic Imaging Modes Contact mode AFM Lateral Force Microscopy ( LFM)

Sample laser cantilever quadrant photodetector tip AFM measures x, y, and z dimensions. The AFM works by scanning a silicon or silicon nitride tip, with.

STM / AFM Images Explanations from

Get to the point!. AFM - atomic force microscopy A 'new' view of structure (1986) AlGaN/GaN quantum well waveguide CD stamper polymer growth surface atoms.

Atomic Force Microscop (AFM) 3 History and Definitions in Scanning Probe Microscopy (SPM) History Scanning Tunneling Microscope (STM) Developed.

Atomic Force Microscopy

Atomic Force Microscopy

NATSYCO. microscopy Optical microscopy Electron microscopy Scanning probe microscope.

Scanning Probe Microscopy (SPM) Real-Space Surface Microscopic Methods.

Northwestern University Rod Ruoff Nanotechnology Fracture Mechanics of One- Dimensional Nanostructures.

BMFB 3263 Materials Characterization Scanning Probe Microscopy & Relates Techniques Lecture 5 1.

Methods and Tehniques in Surface Science

TAPPINGMODE™ IMAGING APPLICATIONS AND TECHNOLOGY

Erie’s Lab Zimeng Li Content Questions Feasibility Problems and Solutions Video/Fluid AFM.

FNI 1A1 Scanning Probe Microscopes SPM History of scanning probe microscopes SPM System Overview Piezoelectric Effect Scanning Tunneling Microscope (STM)

Rupture Force Data Lateral force (nA) Lateral force (nN) C) Fibrin fibers, the skeleton of a blood clot, essentially perform the mechanical task of creating.

Protein Adsorption Affects Osteoblast- Glass Adhesion Strength as Measured by Colloidal Probe Atomic Force Microscopy Jackson Cahn Whitman College.

What Makes Nanoscience so Important? Nanoscience What Is It?

Nanonics General SPM The Nanonics SPM Advantage Standard Atomic Force Imaging at the Highest of Resolutions and Quality Coupled with the Unique.

NANO 225 Micro/Nanofabrication Characterization: Scanning Probe Microscopy 1.

Introduction to Atomic Force Microscopy

Feedback-induced instability in tapping mode atomic force microscopy: theory and experiment by O. Payton, A. R. Champneys, M. E. Homer, L. Picco, and M.

AFM. The cantilever holder The cantilever dimensions Tip position.

Common scanning probe modes

5 kV  = 0.5 nm Atomic resolution TEM image EBPG (Electron beam pattern generator) 100 kV  = 0.12 nm.

Atomic Force Microscopy (AFM)

Atomic Force Microscopy Alexander Chew Florida State University BSC5936 April 2005.

1 Cutting it down to size activity. What is Nanoscience? The study of matter on the “nanometer” scale. A nanometer is one billionth (10 –9 ) of a meter.

ABCs of Nano – a big picture of nanoscience, nanotechnology and nanoengineering Dawen Li, Shoieb Shaik, Scott Wehby December 8, 2014.

Donovan Sung EE235 Student Presentation 4/16/08 Chemical identification of individual surface atoms by atomic force microscopy.

Measurement of CNT Based Fibers and Cavities Holly Tourtillott University of Missouri Micro and Nano Systems Engineering Laboratory, University of Arkansas.

Atomic Force Microscope Nanoindentation/Scratching

Imaging Molecular Structures with Atomic Force Microscopy Tyler Flanagan, Unurbat Erdenemunkh Sponsor - (Professor Michael Boyer) Abstract As part of the.

Electrical Characterization of GUMBOS Using Conductive-Probe Atomic Force Microscopy NAVEEN JAGADISH, SERGIO DE ROOY, ATIYA JORDAN, ASHLEIGH WRIGHT, SUSMITA.

Atomic Force Microscopy (AFM)

Developing Confocal Raman-AFM and Fluorescence-AFM Imaging Techniques to Visualize Drug-Cell Interactions with Further Implications in Cellular Pathology.

Get to the point!.

A Review of Different methods Used in Nano Tissue Engineering

Get to the point!.

Cantilever-based microscopy in biophysics

Modeling Atomic Force Microscopy Helen Tsai Group 10

Scanning Probe Microscopy

Scanning Probe Microscopy History

MultiView 400™ Product Presentation Nanonics MultiView 400™

Modelling of Atomic Force Microscope(AFM)

Scanning Probe Microscopy History

MODULE B-3: SCANNING TUNNELING MICROSCOPY

NANO 230 Micro/Nano characterization

Scanning Probe Microscope

Wei Wang,1 Dong Xiao Niu,2 Xinju Yang1

Force nanoscopy of living cells

Imaging Structural Proteins

Surface ultrastructure and mechanical property of human chondrocyte revealed by atomic force microscopy C.-H. Hsieh, Ph.D., Y.-H. Lin, Ph.D., S. Lin,

The Nano-Scale Mechanical Properties of the Extracellular Matrix Regulate Dermal Fibroblast Function Volker F. Achterberg, Lara Buscemi, Heike Diekmann,

NSOM 2000TS Thomas Erickson.

Nanocharacterization (III)

Volume 96, Issue 9, Pages (May 2009)

Types of Microscopy Type Probe Technique Best Resolution Penetration

Atomic Force Microscope

Atomic Force Microscopy

Volume 86, Issue 5, Pages (May 2004)

Alan Van Orden, Department of Chemistry, Colorado State University

Change in Rigidity in the Activated Form of the Glucose/Galactose Receptor from Escherichia coli: A Phenomenon that Will Be Key to the Development of.

Presentation transcript:

Tomáš Bílý, Martin Šemro Simultaneous AFM and light microscopy of live cells: nano- and microworld within one picture.

Aims of the project Imaging of live cells using AFM and LM Development of software tools for cross calibration of the two imaging platforms

Light microscopy (LM) Imaging of cells in vivo Atomic force microscopy (AFM) Surface topography of living cells Atomic resolution Mapping of force: elasticity, adhesion

Lung epithelial cell H441 major player in lung’s ion balance and liquid clearance proceses

Principle of AFM Cantilever with tip Piezoelectric scanner Contact mode feedback

Image overlay procedure (AFM on LM) 1.Definition of the tip position in cantilever - minimal fail overlay: 1 px (LM 20x) = 6 px (AFM) - maximal fail overlay 1 px (LM 20x) = px (AFM) 2.Overlay of the images based on the shape of the object a) high resolution topography b) force volume mapping 3.Overlay images by specific shape of object

2. Image overlay by shape of an object A) Shape of the cell is different in AFM and LM

2. Image overlay by shape of an object B) Images recorded in the AFM force volume mapping mode - overlay still problematic, yet slightly better - Young’s Modulus on Cell 420 Pa – 20 kPa surroundings 20kPa – 794 kPa (example - proteins 0.5 GPa, wood 1GPa) Overlaid picture (LM and AFM slope)

3. Image overlay by specific shape of object At best nanometer spherical diameter particles – Quantum dots or other nanoparticles (gold) – Precise determination of the centre of an object in LM and AFM

We would like to acknowledge to David Kaftan for leading of this project Thank you for your attention