Biosensors And how they are changing the world

What are they? A device that utilizes biological components to indicate the amount of a biomaterial

Breakdown of Components. Bioreceptor- detects analyte Transducer- converts into readable form Reader Device- displays information

Biosensors in the Future What should a biosensor be? Portable, easy to use, small Easy to be manufactured in mass quantities Low cost, affordable Rapid results Satisfy a large market

Military Use The military has funded research into biosensors Mine sensing bacteria Monitoring gasses on the battlefield Detecting air-borne pathogens To counter Bio-terrorism

Agriculture Detecting heavy metal ions, arsenic, ammonia in water sources Brazilian researchers developing a pesticide detector

Medical Detecting bladder cancer and lyme disease “Artificial Skin” using flexible biosensors that can aid in prosthetics Glucose and Pregnancy Tests Cholesterol Test was a failure

Design Project Factor VIII producing E. coli

What is Hemophilia A Hemophilia affects the ability of the body to control blood clotting or coagulation Hemophilia A is a genetic blood disorder that is caused by a Factor VIII deficiency Hemophilia affects more than 17,000 people in the United States

Current Treatment Options Replacement therapy Desmopressin Antifibrinolytic Medicine

Proposal Using E. coli, create a topical creme containing bacteria engineered to produce clotting factor VIII in order to help close external wounds for people with Hemophilia A

LuxS ABC Transporter Al-2 ATP ADP LsrR P P Factor VIII ATP ADP Factor VIII molecules Export ATP ADP LuxS Porcine Coagulation Factor VIII LSR Transport Cassette Fibrin

Advantages Low Risk, using Pig Factor VIII No chance of blood-transmitted disease Works even with antibodies and inhibitors Site Specific No Injection required

Improvements Kill Switch Does not solve internal bleeding Separate design needed for Hemophilia B