Oral Spot Practical Examination (OSPE) - 2
Shigella on Deoxycholate citrate agar (DCA)
Salmonella on Salmonella-Shigella (SS) agar.
API-20 strip test
Proteus: Urease positive
Differences between Klebsiella, Enterobacter, Serratia Character Klebsiella Enterobacter Serratia Motility - + Indole Red pigment Quellung reaction
Widal tube agglutination test
Interpretation of Widal test Condition Doubtful Significant Typhoid Fever 1:160 1:320 Paratyphoid Fever 1:80
Colicine typing of Shigella
Swarming of Proteus
Pseudomonas on Blood agar
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CETRIMIDE AGAR: Selective for P. aeruginosa
Positive Citrate test for Pseudomonas
Pseudomonas pyocine typing
Bacteroides fragilis: Neomycin selective medium
Bacteroides sensitive to metronidazole disc
Campylobacter Gram negative smear
Campylobacter on Butzler medium
C. jejuni hydrolyze hippurate
Gram stain of Vibrio cholerae
TCBS Agar - Vibrio
Alkaline peptone water and Selenite - F
V. Cholerae : Cholera red reaction
Haemophilus culture on chocolate agar
Haemophilus influenzae: X and V factors
Carbon dioxide candle jar
Fluorescent antibody stain.
Pernasal swabs
Bordetella on Bordet - Gengou medium
Bordetella on selective charcoal cephalexin blood agar
Slide latex agglutination test with specific antisera
Yersinia bipolar safety pin Giemsa staining
Yersinia: non-lactose fermenter on Mc Conkey
Francisella tularensis: on chocolate cysteine agar
Pasteurella multocida: non-hemolytic, mucoid colonies
Blood culture bottles
Brucella: Blood culture Castaneda diphasic bottle
Brucella on tryptone soya agar
B. abortus & meletensis grow in presence of basic fuchsin
Brucella : Standard Agglutination Test
Identification of Brucella species CO2 H2S (days) Growth in presence of Urease 1 2 3 4 5 Basic fuchsin Thionin Methyl violet B. melitensis - + + / - B. abortus B. suis
Blood wet preparation for Borrelia
Leptospira interrogans
Darkfield microscope
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Treponema pallidum
Treponema pertenue
Treponema carateum
VDRL TEST
Treponema Pallidum Immobilization
Treponema pallidum Hemagglutination test
Comparison of VDRL AND RPR Specimen Only serum used, serum heated Serum & plasma used, no heating Antigen Diluted before use Not diluted Reading of results Microscopically from slide Macroscopically from card Technique Difficult Easy Sensitivity Less sensitive More sensitive False positive Less often More often
Legionella pneumophila: Gram stain
L. pneumophila on selective Mueller Hinton agar
Immunofluorescence Microscope
Branching rods of Nocardia asteroides
Gram-positive branching rods of Actinomyces
Sulpher granules of Actinomyces
Disc diffusion sensitivity test
Control Organism Test Organism
E - TEST SENSITIVITY TECHNIQUE
Rosco Neo-Sensitabs Sensitivity Test
Antibiotics synergy test on Mueller–Hinton agar
Antibiotics antagonism on Mueller–Hinton agar
Chlamydia - Giemsa stain
Chlamydia Mc Coy tissue culture
Chlamydia - CFT
Mycoplasma agar: Colonies have fried egg shape
Mycoplasma : A positive cold agglutinin test
Sites of inoculation of fertile eggs Coxiella: Culture in yolk sac of chick embryo
Giemsa stain: Rickettsia inside the cell
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Rickettsia: Weil-Felix Test
Helicobacter pylori: Spiral gram negative rods
Helicobacter pylori on selective Butzler medium
Water Examination: The multiple tube technique
Water Examination: Viable plate count
Milk Examination: Methlylene blue reduction test
Milk Examination: Milk ring test:
Milk Examination: Phosphatase test: