Culturing bioluminescent bacteria dead fish, i love you, but you’re bringing me down.

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Presentation transcript:

culturing bioluminescent bacteria dead fish, i love you, but you’re bringing me down

bacterial bioluminescence Is interesting Diverse Vibrios in the sea Goal: to isolate bioluminescent bacteria for further study

strategy Bioluminescent bacteria are present throughout marine environments Bioluminescence is detectable when cells are present at high density Therefore: make bacteria grow to induce luminescence, pick them out

Seawater complete media –Artificial seawater, bacto tryptone, yeast extract, glycerol, agar

Photo courtesy S. Russell

How do we improve yields? Sample from enriched locations Improve culturability (great plate count anomaly) –In situ culturing with diffusion chambers? D’Onofrio et al (2010) Chemistry & Biology. 17(3):

How to improve yields? (cont’d) More selective media, or bait? –chemotaxis –Non-symbiosis-related functions of bioluminescence? Improve detection on plate

the end