Blood Smear.

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Presentation transcript:

Blood Smear

This peripheral blood smear from a patient with essential thrombocythemia shows increased numbers of platelets, including some large forms. (H and E, 400x)

Malaria

Atypical, or reactive, lymphocytes

Acanthocytes (red arrow) and schizocytes (black arrow) in a canine blood smear

Keratocytes in a canine blood smear

Band neutrophil

Vacuolated monocyte

Lymphocyte with azurophilic granules

Hypersegmentation

Toxic granulation

Döhle body

Platelets - finger-stick

Giant platelet

Nucleated Red Blood Cells

Macrocytic Red Blood Cells Compared to Normal Red Blood Cells

Promyelocyte

Segmented Neutrophil, Metamyelocyte, Band

Sickle Cell Anemia

Pernicious Anemia

Plasma cell

Hairy cell

Larger than average RBCs are macrocytic (left), while those smaller than average are microcytic (right).

Pale cells (central pallor >1/3 dia) are referred to as hypochromic (right), while cells without central pallor are called hyperchromic (left).

SCHISTOCYTES

TARGETS

OVALOCYTES

STOMATOCYTES

SPHEROCYTES

ACANTHOCYTES

BURR CELLS

Elliptocytosis

Tear Drop Cells

Rouleaux Formation

Basophilic stippling

Howell-Jolly Bodies

Platelet Clumping

Auer Rods

With iron deficiency anemia, the MCV of the red blood cells is decreased, the zone of central pallor is increased, and the overall sizes and shapes of the RBC's are less uniform (increased anisocytosis and poikilocytosis ).

Heinz bodies (red arrows) and nucleated red blood cells (purple arrows) in scattered erythrocytes of a dog with zinc toxicosis (Dog, blood smear, Wright-Leishman stain).

Spherocytes (red arrows) and nucleated red blood cells (purple arrows) in the blood smear of a dog with zinc toxicosis (Dog, blood smear, Wright-Leishman stain).

Can you identify the segmented neutrophil, band neutrophil, lymphocyte, monocyte, eosinophil, basophil, and platelet in this image?

This is a picture of a blood smear as seen by a microscope This is a picture of a blood smear as seen by a microscope.  The red cells are the smaller, more numerous round objects. There are two white cells in the middle (they are blue because they have been stained).

Picture of bone marrow smear (control); Normal granulocytes and erythroblasts are evident.

Acute lymphoid leukemia (ALL); There is a marked proliferation of small lymphoblasts.

Acute myeloid leukemia (AML); There is a marked proliferation of large myeloblasts

Chronic myeloid leukemia (CML); There is a marked proliferation of granulocytes at various stages of maturation.

AML (M0)

AML (M1)

AML (M2)

AML (M3)

AML (M4)

AML (M5)

AML (M5) -alpha-naphthyl butyrate esterase and chloroacetate esterase stains

AML (M6) -PAS stain

AML (M6)

AML (M7)

Chronic myelocytic leukemia

Chronic lymphocytic leukemia (CLL)

Acute Lymphoblastic Leukemia (ALL)

Acute lymphocytic leukemia (ALL)

ALL-L1

ALL-L2 

ALL-L3

thanks