Place, date, unit, occasion etc. Slide 1 Molecular Immunology at IGM Who? Where? When? Why and What for? Gudrun Weiss

Place, date, unit, occasion etc. Slide 2 DTU KU LIFE Where? When? 5. floor at IGM at the end of “højhuset” January 2009

Place, date, unit, occasion etc. Slide 3 Work Flow Hanne Frøkiær Continous Non Stop Process Transformation Process 1 Associate Professor 1 Post Doc 2 PhD Students 2 Lab Technicians 1 Lab Tech Trainee Molecular Immunology Group

Place, date, unit, occasion etc. Slide 4 Molecular Immunology Group Stine Hanne Louise Kristina Who?

Place, date, unit, occasion etc. Slide 5 The Real Engine of the Lab Anni Shadi Marianne

Place, date, unit, occasion etc. Slide 6 PhD Student Kristina Galathea Expedition Marine Bacteria Collaboration with DTU Aqua Immune stimulatory effects?

Place, date, unit, occasion etc. Slide 7 Kristina's Winter

Place, date, unit, occasion etc. Slide 8 Associate Professor Stine DNA RNAProtein miRNA (micro RNA) Small non coding RNA -- ug c ug a u au uua auc cuu guaaaggcgugu uug a gg gu c gu c gaccguag u ua aau uag gaa cauuucugcaca aac a 5’ 3’ Regulation of Biological Processes

Place, date, unit, occasion etc. Slide 9 PhD Student Louise Uptake of bacteria in immune cells Endocytosis

Place, date, unit, occasion etc. Slide 10 Research Topic: Immunological Recognition and Response to Microorganisms How are Microorganisms recognized? Which Cells interact with Microbes? What determines the Response to Microbes ? How is a Microbe recognized as a Pathogen ? Special Focus: Gastro-Intestinal Tract

Place, date, unit, occasion etc. Slide 11 Why and What for?

Place, date, unit, occasion etc. Slide 12 The Gastrointestinal Tract One of the key organs in the body Large surface area : m 2 (skin: only 2 m 2 ) Surface small intestine: Size of a Tennis Court

Place, date, unit, occasion etc. Slide 13 The Human Microbiota Human Gastro-Intestinal Tract ~ 1x10 14 bacteria, several thousand different species 10 x more bacteria cells than human cells 100 x more bacteria genes than human genes

Place, date, unit, occasion etc. Slide 14 - “ Pro Bio ” – “For life” - Gram positive Lactic Acid Bacteria - Lactobacilli - Bifidobacteria - Natural inhabitants of the intestine, “ Friendly Bacteria ” -Beneficial Effects : Infectious diarrhea (especially rotavirus) Inflammatory bowel disease, Lactose intolerance, Allergies Probiotics

Place, date, unit, occasion etc. Slide 15 Intestinal Tract

Place, date, unit, occasion etc. Slide 16 Dendritic Cells Excretion of immunstimulatory factors Immune response in the body

Place, date, unit, occasion etc. Slide 17 + Growth factor GM-CSF Stimulation Assay 8 days: Immature Dendritic Cells Proteins: Supernatant ELISA Genes: Cells Real-Time PCR Microarray

Place, date, unit, occasion etc. Slide 18 How do Probiotic Bacteria stimulate the Immunsystem? Up/Downregulationof Genes in Dendritic cells Elucidation of Pathways Lactobacillus acidophilus Bifidobacterium bifidum

Place, date, unit, occasion etc. Slide 19 Lactobacilli Bifidobacteria Pro-inflammatory Anti-inflammatory

Place, date, unit, occasion etc. Slide 20 Inhibition of pro-inflammatory interleukin IL-12 - Lactobacillus Acidophilus - Bifidobacterium Bifidum ELISA Proteins Mechanisms? MICROARRAY

Place, date, unit, occasion etc. Slide 21 L. acidophilus NCFM Response to Virus Microarray Analysis Screening: > Genes

Place, date, unit, occasion etc. Slide 22 Activation of the Viral Immune System Expression of the key regulator: Interferon β (IFN- β) Protection against influenza?!?

Place, date, unit, occasion etc. Slide 23 Lactobacillus acidophilus Virus + Pathogenic bacteria JNK pathway Mechanisms – Use of Knock Out Mice ? dsRNA ssRNA DNA

Place, date, unit, occasion etc. Slide 24 My Winter

Place, date, unit, occasion etc. Slide 25 General Atmosphere in the Lab?

Place, date, unit, occasion etc. Slide 26 Paper rejected 

Place, date, unit, occasion etc. Slide 27 Paper accepted

Place, date, unit, occasion etc. Slide 28 Have a nice Weekend!!! Any Questions???