Biochemistry Lecture 1. Bloom’s Taxonomy Richard C. Overbaugh, Lynn Schultz Old Dominion University.

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Presentation transcript:

Biochemistry Lecture 1

Bloom’s Taxonomy Richard C. Overbaugh, Lynn Schultz Old Dominion University

Student Objectives for this course Calculate bioenergetic parameters and evaluate carbon molecules reactions Reproduce and explain key metabolic processes: glycolysis, TCA cycle, electron transport and oxidative phosphorylation, fatty acid oxidation Analyze protein structure and function and evaluate different methods used to assess and test structure and function Trace key metabolites through key pathways, design experiments to test carbon flux Compare and contrast modes of metabolism regulation and judge the effects of different modes

Biochemistry is the chemistry of Living Systems The Chemistry of Carbon and Water Themes for this course: –The transformation of energy –Levels of complexity

Cells

Levels of Complexity Lipids

The Inner Life of the Cell ife.htmlhttp://multimedia.mcb.harvard.edu/anim_innerl ife.html

Why Carbon BondStrength (kJ/mol) C – C347 – 356 C = C611 C = C837 C – O336 C – H356 – 460 Si – Si230 Si – O368 O – O146 O = O498 N – N163 N = N418 N = N946 What can we learn from this? C – C bond is stronger than C – O –Stable in oxygen rich environment! Two C – C bonds are stronger than one C = C –Chains are stable! C – H bond is strong –Hydrocarbons stable at room temperature!

Important Functional Groups Alcohol Thiol Amine Ether Thioether Peroxide Disulfide Aldehyde Ketone Carboxylic acid Ester Anhydride Amide Thioester Phosphate Phosphoester Phosphoanhydride

Carbon and Functional Groups

Other biomolecules NADP+ PEP Phosphatidylcholine

Bioenergetics Cell ReactantsProducts Steady State = constant flux Structural differences between reactants and products Concentration differences between reactants and products

Water

Hydrogen Bonds

Water

pH pH = -log[H + ]

Acids

Buffers

HA H + + A -   Hendeson Hasselbalch Equation

Summary Biochemistry is the chemistry of living things –Which is the chemistry of carbon and water Carbons unique bonding properties Water: hydrogen bonds and ionization Buffers and pH

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