MECHANIZATION OF LILY MICROBULB MULTIPLICATION OPERATIONS Ta-Te Lin and Ching-Lu Hsieh Department of Agricultural Machinery Engineering, National Taiwan University, Taipei, Taiwan, ROC

MECHANIZATION OF LILY MICROBULB MULTIPLICATION OPERATIONS Ta-Te Lin and Ching-Lu Hsieh Department of Agricultural Machinery Engineering, National Taiwan University

n INTRODUCTION n MODELING n PROCESS OPTIMIZATION n MECHANIZATION OF MULTIPLICATION PROCESS n CONCLUSIONS

INTRODUCTION n Lily microbulb tissue culture cycle n Microbulb dissecting and transplanting

LILY MICROBULB TISSUE CULTURE CYCLE

LILY MICROBULB IN CULTURE VESSEL

MICROBULB DISSECTING AND TRANSPLANTING n Vessel opening n Bulb gripping n Root and leaf removal n Bulb grading n Bulb scale separation n Scale transplanting n Vessel sealing n Vessel labeling

MODELING n Analysis of manual operation n Batch process model n Stepwise process model

ANALYSIS OF MANUAL OPERATION

FLOW CHART OF LILY MICROBULB MULTIPLICATION PROCESS

BATCH PROCESS MODEL

STEPWISE PROCESS MODEL

Probability of measured entry vessel quantity with fitted lognormal density function

Probability of measured single vessel processing time with fitted lognormal density function

Probability of measured propagation rate with fitted lognormal density function

Total processing time, as affected by entry vessel quantity under various single vessel processing times (ST)

Finished vessel quantity, as affected by entry vessel quantity under various multiplication rates (MR)

Contour plot of predicted finished vessel quantities (dotted lines) and total processing times (solid lines)

PROCESS OPTIMIZATION n Response surface method (RSM) n Optimum analysis

RESPONSE SURFACE METHOD n Experimental design n Parameter estimation n Reliability test n Response surface examination

EXPERIMENTAL DESIGN n Dependent variables Separation rateSeparation rate Injury rateInjury rate n Independent variables Cutting positionCutting position Spinning speedSpinning speed Separation timeSeparation time

Values of the coded and uncoded independent variables in the RSM analysis of microbulb scale separation operation

Experimental conditions of the Box-Behnken experimental design for RSM analysis and the experimental results

Coefficients of the regressed 2nd order polynomial equations for separation rate and injury rate

Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position A

Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position B

Predicted separation rate (solid line) and injury rate (dotted line) for microbulb of cutting position D

Comparison between predicted and measured separation rate, injury rate and propagation rate of the validation experiment

MECHANIZATION OF MULTIPLICATION PROCESS n Scale separation n Scale transplanting n Other mechanical components

SCALE SEPARATION

Separation rate of lily microbulb with cutting position A as affected by separation time, spinning speed

Separation rate of lily microbulb with cutting position B as affected by separation time, spinning speed

Separation rate of lily microbulb with cutting position C as affected by separation time, spinning speed

Separation rate of lily microbulb with cutting position D as affected by separation time, spinning speed

Injury rate of lily microbulb with cutting position A as affected by separation time, spinning speed

Injury rate of lily microbulb with cutting position B as affected by separation time, spinning speed

Injury rate of lily microbulb with cutting position C as affected by separation time, spinning speed

Injury rate of lily microbulb with cutting position D as affected by separation time, spinning speed

SCALE TRANSPLANTING

CONCLUSIONS n n The bulb scale separation and transplanting operation was identified as the most laborious operation in the process. n n A batch-type model and a stepwise model were constructed to study the influence of operation parameters. n n At an optimum spinning speed and separation time, lily microbulb could be successfully separated into scales with acceptable injury rate. n n A bulb scale separation and transplanting machine was developed and the process was optimized.

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