10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes1 10/17/05 Genes & Genomes (formerly Gene Prediction - 1)

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10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes1 10/17/05 Genes & Genomes (formerly Gene Prediction - 1)

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes2 Gene Prediction & Regulation Gene structure & regulation Eukaryotes vs prokaryotes Methods for predicting genes Predicting regulatory regions Predicting miRNAs & targets

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes3 Reading Assignment (for Mon) Mount Bioinformatics Chp 9 Gene Prediction & Regulation pp Eukaryotic vs Prokaryotic Gene Structure Ck Errata: Brown Genomes 2 (NCBI textbooks online)NCBI textbooks online) Sect 7 Understanding a Genome Sequence - Overview Sect 7.1 Locating Genes in a Genome Sequence

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes4 Reading Assignment (for Wed) Mount Bioinformatics Chp 9 Gene Prediction & Regulation pp Predicting Promoters Ck Errata: Brown Genomes 2 (NCBI textbooks online)NCBI textbooks online) Sect 9 Overview: Assembly of Transcription Initiation Complex Sect DNA binding proteins, Transcription initiation

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes5 Eukaryotes vs Prokaryotes Eukaryotic cells are characterized by membrane-bound compartments, which are absent in prokaryotes. “Typical” human & bacterial cells drawn to scale. BIOS Scientific Publishers Ltd, 1999 Brown Fig 2.1

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes6 Central Dogma - “old” BIOS Scientific Publishers Ltd, 1999 Brown Fig 3.2a

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes7 Central Dogma - “new” BIOS Scientific Publishers Ltd, 1999 Brown Fig 3.2b

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes8 Central Dogma - “new” BIOS Scientific Publishers Ltd, 1999 Brown Fig 3.2b

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes9 Central Dogma - “new” BIOS Scientific Publishers Ltd, 1999 Brown Fig 3.2b

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes10 A very simple eukaryotic gene BIOS Scientific Publishers Ltd, 1999 Brown Fig 1.17

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes11 Comparison of Gene Structures BIOS Scientific Publishers Ltd, 1999 Brown Fig 2.2

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes12 Comparison of Gene Structures BIOS Scientific Publishers Ltd, 1999 Brown Fig 2.2

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes13 Comparison of Gene Structures BIOS Scientific Publishers Ltd, 1999 Brown Fig 2.2

10/17/05 D Dobbs ISU - BCB 444/544X: Genes & Genomes14 DNA Interactive: "Genomes" A tutorial on genomic sequencing, gene structure, genes prediction Howard Hughes Medical Institute (HHMI) Cold Spring Harbor Laboratory (CSHL)