RE digests & RE maps OD and uses Last class How to read a paper Intro to Paper 1.

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Presentation transcript:

RE digests & RE maps OD and uses Last class How to read a paper Intro to Paper 1

Purifying proteins Purification – based on what properties?

Purifying proteins - Solubility [Salt] Fraction protein in precipitate

Ammonium sulfate precipitation

Getting rid of salt: Dialysis

Ammonium sulfate precipitation 0-40% 40-60% 60-80% Bradford assay & enzyme assay (why)? - Every fraction Lab Dialysis

First evidence of PA-GFP…

Increase in fluorescence of PA-GFP…

pH affects

Photostability

OK – what about in cells?

Can we study dynamics?

Lets do a real experiment – live trafficking

Following live trafficking of proteins

Lysosomal trafficking needs microtubules