Jacob Frye, Courtney Green, Christopher Robertson, and Scott Shields

Introduction  Water is the most important resource.  chemicals(chlorine, iodine) are widely used for sterilization.  Development of alternative sterilization using electricity.

Background  Electroporation – forcefully opening pores of bacteria.  Electrical team performed static electroporation tests.  Results more inclined towards repetition and low voltage.

Background cont.  Use of E. coli k12 to minimize risk while properly modeling bacteria response to electroporation.  Group certification to handle high voltage sources.

Escherichia coli K12

 Safe and Harmless.  Similar Properties to Common or Lethal E. Coli  Optima Growth at 37 degrees Celsius  Kill rate exists at 42 degrees Celsius

Biosafety Qualifications  To work in the biological lab, Scott and Jacob had to pass the biosafety lab examination and read the Biosafety lab manual  Strict guidelines for the type of clothing and to properly test E. coli K12 around other dangerous bacteria.

Grow Process of E. coli K12  Plate preparation  Growing process  Incubation period  Treatment and testing  Growing treated bacteria  Determine bacterial reduction

Specific Phases of E. coli K12  Lag Phase  Log/Exponential Phase  Stationary Phase  Death Phase

Design Parameters  Use results from previous electrical team to develop mechanical flow system.  100% kill rate of bacteria  5 kV/cm³ to kill bacteria  Attain an 8 oz. cup of drinkable water in under 10 minutes

Figure 1: Primary Design of Fluid Chamber

Figure 2: Secondary Design (Implementation Pending Time Constraints)

Figure 3: Peristaltic Pump used to generate flow

Manufacturing  Prefabricated Acrylic Rectangle Tubing  Lathe turned connectors  16 gauge silver sheet  8 gauge silver rod lathe turned ends  Interference press fit connection  Silicon adhesive for water containment

Figure 4: Fluid Chamber with silver plates connected to Peristaltic Pump

Preliminary Testing  Determine the possible flow rates with the Peristaltic Pump.  Tested the chamber at maximum flow rate.  Tested the chamber at our design flow rate.  Video and Still Images to follow.