Final stuff: n Lab practical: Apr 29 n Final exam: due Fri May 2:15

Today n IDEM sampling n Assessment exercise

MACROINVERTEBRATE COMMUNITY MONITORING EXAMPLE INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT

STAGES OF BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

What are We Trying to Measure ??

“The ability of an aquatic ecosystem to support and maintain a balanced, integrated, adaptive community of organisms having a species composition, diversity, and functional organization comparable to the best natural habitats within a region.” BIOLOGICAL INTEGRITY

STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

Aquatic Larvae Aquatic Adults Terrestrial Adults

Adult and Larval Caddisflies (Trichoptera)

USEPA Rapid Bioassessment Protocols Riffle Habitats

Two Composite KICK Screen Samples

STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

Sample Elutriation  Remove Gravel and Sand  Remove Excess CPOM

The Final Sample Ready for Preservation

Field Preservation of Samples

GPS for Spatial Reference PAPERWORK…PAPERWORK…. TRIPLE REDUNDANCY  Water Chemistry  Habitat Assessments  Field Observations

STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

All Samples are Returned to Laboratory for Processing

Squares (25 cm 2 ) Removed by Suction to Petri Dish 100 Organism Sub-Sample 15 Minute Sort Sub-Sample

100 Organism Sub-Sample Selected at Random Second Sub-Sample 15 Minutes Entire Pan “Randomly” Sorted Using Forceps Final Sort Under Microscope

Sub-Samples Processed to Family Level

All Samples Preserved for Permanent Museum Storage

STAGES OF A BIOLOGICAL COMMUNITY ASSESSMENT n n DATA REDUCTION AND ANALYSIS FIELD PROCESSING n LABORATORY PROCESSING n SAMPLE COLLECTION

TEN SUB- METRICS SITE INFORMATION TAXONOMIC INFORMATION AND TAXA COUNTS LABORATORY BENCHSHEET

Example of 2 Sub-Metrics E P T EPT Index = 6 Families EPT Count = 7 Individuals Metric Scores = 0, 2, 4, 6, 8

Scoring Criteria for Riffle KICK Samples

Not All Rivers and Streams Have Riffle Habitats

Multi-Plate Samples are Collected Individually Multi-Plate Samples are Preserved in the Field and Scraped in the Laboratory

Systematic Community Assessments of Benthic Macroinvertebrate Communities are Effective SUMMARY

IBI -- Family Level Multi-Metric Indices Provide: n n Cost Effective Data of Ecological Health of Rivers and Streams n n n n Assessment Information to Make Management Decisions

Lammert & Allen 1999 Scoring Classification Metric135 Total Taxa< > 30 Mayfly Taxa > 6 Caddisfly Taxa > 5 Dipteran Taxa > 6 Relative Abundance of Omnivores 70 – 100 %57 – 69 %43 – 56 % Relative Abundance of Filterers 51 – 69 %50 – 31 %9 – 30 % Relative Abundance of Grazers 0 – 19 %20 – 32 %> 33 % Relative Abundance of Predators 24 – 33 %14 – 23 %2 – 13 % Dominance68 – 88 %47 – 67 %24 – 46 %

Actual Observation IBI Score (no.) Total Taxa 191 Mayfly Taxa 21 Caddisfly Taxa 21 Dipteran Taxa 31 Relative Abundance of Omnivores Relative Abundance of Filterers Relative Abundance of Grazers Relative Abundance of Predators Dominance B-IBI score Lammert & Allan (1999)Total IBI21