Anatomy I: Human Anatomy Spring Semester 2010 Spring Semester Units 5 Units Lecture: Tues Thurs 5:30-6:45, Room 1814 Lecture: Tues Thurs 5:30-6:45, Room 1814 Lab: Tues Thurs 7:00- 9:50, Room 1810 Lab: Tues Thurs 7:00- 9:50, Room 1810

John Gallagher, BS, MS, DVM Colorado State University Colorado State University –BS, Animal Sciences, 1974 –MS, Clinical Sciences, 1976 –DVM, Veterinary Medicine, 1980

Turn off your Cell Phone! No texting, either. Review the new Safety Rules and microscope guidelines in the syllabus. Don’t bother to telephone me. is much better! My web site: Blackboard: A Few Details:

The Books: Marieb, Mallatt, Wilhelm, Human Anatomy, Pearson, Benjamin Cummings, 5 th ed., 2008 Marieb, Mallatt, Wilhelm, Human Anatomy, Pearson, Benjamin Cummings, 5 th ed., 2008 Marieb and Mitchell, Human Anatomy & Physiology Lab Manual, Cat Version, 9th edition, Marieb, 2008 Marieb and Mitchell, Human Anatomy & Physiology Lab Manual, Cat Version, 9th edition, Marieb, 2008Optional: Kapit and Elson, The Anatomy Coloring Book Kapit and Elson, The Anatomy Coloring Book

First Assignment! Send me an before Sunday, 11:00 PM, the first week of class (3 points!) Always put Anatomy in the subject line. Always put Anatomy in the subject line. –Why are you taking this class? –What is your college background? –Do you have a job? Where? –What special interests do you have?

Anatomy = The study of external and internal structures The study of external and internal structures The physical relationships among body parts The physical relationships among body parts Temnein (Gr.) = cut Temnein (Gr.) = cut Leonardo da Vinci

Organization Atoms Atoms Molecules and Molecules and Macromolecules, such as proteins Macromolecules, such as proteins Organelles Organelles Cells Cells Tissues Tissues Organs Organs Organ Systems Organ Systems Organism Organism

The Systems

Planes Frontal Frontal –Think front! Transverse Transverse –“ Trans” = across Sagittal Sagittal –Especially mid-sagittal Oblique Oblique –Any Diagonal

Quadrants Surrounding the Umbilicus RUQ: Gall Bladder, Rt kidney RUQ: Gall Bladder, Rt kidney RLQ: Appendix, Rt ovary RLQ: Appendix, Rt ovary LUQ: Lt liver lobe, stomach, Lt kidney LUQ: Lt liver lobe, stomach, Lt kidney LLQ: Small intestine, Lt ovary LLQ: Small intestine, Lt ovary

These words are used all the time, including this class! Note the Anatomic Position.

Get used to them! Superficial: Toward the skin Deep: Farther inside Medial: Toward the midline Lateral: Away from the midline

Body Cavities

Body Cavity Membranes More later!

Body Cavities

1. no open-toe or open-heel shoes allowed in the labs; 2. no eating or drinking (including WATER), nothing by mouth in the labs EVEN if it is only lecture, no fingers in mouth or eyes (contact replacement, taking medication, etc); 3. nothing down the drains, but water (and hand & dish detergents/soaps); 4. nothing into the regular trash cans but paper towels from hand-washing and clean office paper. Lab Safety (details in syllabus)

Medical Imaging Light Microscopy (cytology) Light Microscopy (cytology) Electron Microscopy (ultrastructure) Electron Microscopy (ultrastructure) –TEM –SEM Radiography (X-Rays) Radiography (X-Rays) –CT Scanning Ultrasound Ultrasound Positron Emission Tomography (PET) Positron Emission Tomography (PET) Magnetic Resonance Imaging (MRI) Magnetic Resonance Imaging (MRI)

Light Microscopy

Light Microscopy, cont’d 1. The specimen is fixed 1.Usually in 10% formalin 2. Then embedded in paraffin 3. Sectioned with a microtome 1.Approx 5 μ sections 4. and stained 1.Enhances contrast for better visualization 2.Many types of stains 1. H & E = Hematoxylin and Eosin 2. May add “artifact”

Concepts Important for Viewing Resolution - The distance between two objects that is required for the two objects to be distinguished. Depth of Field - depth that focus is clear Contrast Formation - (e.g. absorption contrast) Illumination Source - diascopic vs. episcopic from below (compound) vs. from above (dissecting) Artifact – Distortion from preparation of the specimen

How The Concepts Interact As Resolution and Brightness improve, Depth of Field and Contrast are diminished. Vice versa is also true. As magnification improves, resolution improves Contrast can be adjusted by the iris diaphragm, the position of the condensor, and the magnification.

Electron Microscopy Uses a beam of electrons (instead of visible light) to view topography, morphology, composition, and crystallography. EM was developed for 10,000 – 25,000 X magnification. Properties of light limit magnification of light microscopes to 1000 X and resolution to 0.2  m. Transmission Electron Microscope