Cellular Pathways Metabolic pathways occur in small steps, each catalyzed by a specific enzyme. Metabolic pathways are often compartmentalized and are.

Slides:



Advertisements
Similar presentations
STAGE 4: ELECTRON TRANSPORT AND CHEMIOSMOSIS (Figs – P ) NADH and FADH 2 eventually pass H-atom electrons to the electron transport chain.
Advertisements

How Cells Harvest Energy Chapter 7. 2 Respiration Organisms can be classified based on how they obtain energy: autotrophs: are able to produce their own.
Cellular Pathways that Harvest Chemical Energy
Cellular Respiration Notes: 10/8/12.
Respiration The Four Stages.
Lesson 7: Harvesting of Energy “Cellular Respiration”
Cellular Respiration Pp 69 – 73 &
CHAPTER 9 CELLULAR RESPIRATION: HARVESTING CHEMICAL ENERGY
Cellular Respiration: Harvesting Chemical Energy
Cellular Respiration.
Cellular Respiration 7.3 Aerobic Respiration.
Energy Releasing Pathways ATP
Cellular Respiration AP Biology Unit 4 Metabolic Pathways Metabolism = Totality of an organism’s chemical reactions Ex. Heme Synthesis Case Studies --
The Electron Transport Chain. Oxidative Phosphorylation Oxidative Phosphorylation is the indirect formation of ATP. It involves a series of redox reactions.
Cellular Respiration: Harvesting Chemical Energy.
Stage 2: Pyruvate Oxidation Products: acetyl-CoA, NADH, CO 2, H+ (twice) mnm.
 Organisms must take in energy from outside sources.  Energy is incorporated into organic molecules such as glucose in the process of photosynthesis.
Chapter 9 Cellular Respiration. I CAN’S/ YOU MUST KNOW The difference between fermentation & cellular respiration The role of glycolysis in oxidizing.
Cellular Respiration Stage 4: Electron Transport Chain
The Krebs Cycle Biology 11 Advanced
Aerobic Cellular Respiration
AP Biology Ch. 9 – Cellular Respiration. Catabolic pathway Fermentation Aerobic respiration Anaerobic respiration Cellular respiration Redox reaction.
Electron transport chain Cellular respiration is a series of reactions that: -are oxidations – loss of electrons -are also dehydrogenations lost electrons.
CELLULAR RESPIRATION: HARVESTING CHEMICAL ENERGY The Process of Cellular Respiration 1.Respiration involves glycolysis, the Krebs cycle, and electron transport.
Cellular respiration: Harvesting chemical energy.
How Cells Harvest Energy Chapter 6
How Cells Harvest Energy
7 Energy and Electrons from Glucose The sugar glucose (C 6 H 12 O 6 ) is the most common form of energy molecule. Cells obtain energy from glucose by the.
Chapter 7: Cellular Pathways That Harvest Chemical Energy CHAPTER 7 Cellular Pathways That Harvest Chemical Energy.
Chapter 7: Cellular Pathways That Harvest Chemical Energy Cellular Pathways That Harvest Chemical Energy Obtaining Energy and Electrons from GlucoseObtaining.
Pathways That Harvest Chemical Energy
Aerobic Respiration + The 1980s? Check it out! Check it out!
Cell Respiration C 6 H 12 O O H 2 O  6 CO H 2 O + ATP.
Cellular Respiration: Harvesting Chemical Energy
Pp 69 – 73 & Define cell respiration Cell respiration is the controlled release of energy from organic compounds in cells to form ATP Glucose.
2.2 Cellular Respiration: The Details
Respiration. Cellular respiration — glucose broken down, removal of hydrogen ions and electrons by dehydrogenase enzymes releasing ATP. The role of ATP.
Glycolysis, Kreb’s, and ETC
Aim #38: How do our cells carry out aerobic cellular respiration?
UNIT III – CELLULAR ENERGY
Respiration occurs in three metabolic stages: glycolysis, the Krebs cycle, and the electron transport chain and oxidative phosphorylation. Respiration.
Chapter 07 Cellular Respiration Biology II – Dual Enrollment.
Cellular Respiration Making ATP. Cellular Respiration Cell respiration is the controlled release of energy from organic compounds in cells to form ATP.
Aerobic Respiration Section 9:2. Overview Krebs Cycle: In the presence of O2, Pyruvic Acid oxidizes, the reduction of NAD + to NADH, and FAD to FADH,
C 6 H 12 O O H 2 O  6 CO H 2 O + ATP.
Ch. 6: Cellular Respiration Harvesting Chemical Energy.
School of Sciences, Lautoka Campus BIO509 Lecture 27: Respiration
CHAPTER 9 CELLULAR RESPIRATION: HARVESTING CHEMICAL ENERGY
Cellular Respiration & Fermentation
Cellular Respiration.
How do cells extract energy from glucose?
Aerobic Respiration SBI4U1.
Where did Bruce Lee get all that energy from?
Topic 8.2 Chap. 7 in Falcon Book
Cellular Respiration: The Details
Pathways that Harvest and Store Chemical Energy
Cellular Respiration & Fermentation
Topic 8.1 Cell Respiration (HL).
Cellular Respiration (continued)
Chapter 2: CELLULAR RESPIRATION
Cell Respiration Topic 2.8 and 8.1.
Chapter 7 Cellular Respiration
Cellular Respiration (continued)
Cellular Respiration (continued)
AP Biology Ch. 9 Cellular Respiration
Cellular Respiration: Harvesting Chemical Energy
Step 4: Electron Transport Chain & Chemiosmosis
Aerobic Respiration Section 9:2.
Aerobic Respiration: Overview
Energy in food is stored as carbohydrates (such as glucose), proteins & fats. Before that energy can be used by cells, it must be released and transferred.
Presentation transcript:

Cellular Pathways Metabolic pathways occur in small steps, each catalyzed by a specific enzyme. Metabolic pathways are often compartmentalized and are highly regulated.

Cellular Respiration Overview Obtaining Energy and Electrons from Glucose When glucose burns, energy is released as heat and light: C 6 H 12 O O 2  6 CO H energy The same equation applies to the metabolism of glucose by cells, but the reaction is accomplished in many separate steps so that the energy can be captured as ATP.

Energy Overview

C 6 H 12 O 6 + 6O > 6CO 2 + 6H 2 O + energy via heat and ATP

O atoms draw shared electron pairs to themselves (oxidation of C). Do the electrons get more/less stable? Exergonic/endogonic?

C 6 H 12 O 6 + 6CO 2  6CO 2 + 6H 2 O + ATP Breaking the bonds between the six C atoms of glucose, results in 6 CO 2 molecules. Moving hydrogen atom electrons from glucose to oxygen, forms 6 H 2 O molecules. As much of the free energy released in the process as possible is trapped as ATP.

Obtaining Energy and Electrons from Glucose As a material is oxidized, the electrons it loses transfer to another material, which is thereby reduced. Such redox reactions transfer a lot of energy. Much of the energy liberated by the oxidation of the reducing agent is captured in the reduction of the oxidizing agent.

Redox Reactions

The ultimate goal of cellular respiration is to capture as much of the available free energy in the form of ATP. This goal is accomplished through 2 different energy-transfer mechanisms: 1.substrate-level phosphorylation 2.oxidative phosphorylation

(translation: sticking on a phosphate, which can later be used to make ATP from ADP) 1. Substrate-Level Phosphorylation (energy invested) ATP is formed directly a phosphate-containing compound transfers a PO 4 - directly to ADP, forming ATP 30.5 kJ/mol of potential energy is transferred.

2. Oxidative Phosphorylation ATP is formed indirectly involves sequential redox reactions, with O being the final electron acceptor

Energy Transfer Subtrate-level Phosphorylation ATP is formed directly in an enzyme-catalyzed reaction. Phosphate containing compound transfers a phosphate group directly to ADP forming ATP. 31KJ/Mol is transferred 50KJ/Mol in living cells Oxidative Phosphorylation Involves sequential redox reactions. O is the final electron acceptor. NAD+ removes 2H from glucose and is reduced to NADH FAD is reduced to FADH 2 FADH2 and NADH move free energy from one place to another.

Energy Carriers NAD + and FAD + are low energy, oxidized coenzymes that act as electron acceptors. When an electron(s) is added to these molecules, they become reduced to NADH and FADH 2. In this case, reducing a molecule gives it more energy.

The coenzyme NAD is a key electron carrier in biological redox reactions. It exists in two forms, one oxidized (NAD + ) and the other reduced (NADH + H + ).

Mitochondria Specialize in the production of ATP. Only in eukaryotic cells. Double membrane – smooth outer layer and folded inner membrane (cristae). Matrix is protein rich and fills the innermost space of the mitochondria. Intermembrane space (between the 2 membranes) Contain their own DNA (mtDNA) which lead to the endosymbiosis hypothesis. Smooth Highly folded Folds of the inner membrane Protein-rich liquid Fluid-filled intermembrane space

The Big Picture The entire process occurs in 4 stages and in 3 different places within the cell: Stage 1: Glycolysis - a 10 steps occurring in the cytoplasm Stage 2: Pyruvate Oxidation - 1 step occuring in the mito. matrix Stage 3: Krebs cycle - 8 steps occuring in the mito. matrix Stage 4: ETC and chemiosmosis - many steps occuring in the mito. cristae

Overview

Energy Pathways

Glycolysis There are 2 main phases of glycolysis: Glycolysis I - activation phase, which uses ATP molecules Glycolysis II - oxidative and phosphorylation reactions, which not only reduce glucose to pyruvate but also produce ATP molecules C 6 H 12 O 6 + 2ADP + 2P i + 2 NAD > 2 pyruvate + 2ATP + 2(NADH + H + )

Stage 1: Glycolysis Occurs in cytoplasm Anerobic and does not require oxygen Glucose is split into two 3-C molecules called pyruvate (pyruvic acid). Transfers only 2.2% of free energy available in 1 mol of glucose to ATP.

 is not a strong enough oxidizer to strip electrons from C-H bonds in glucose at room or body temperature. Enzymes required.Enzymes required Glycolysis: From Glucose to Pyruvate Glycolysis is a pathway of ten enzyme- catalyzed reactions located in the cytoplasm. It provides starting materials for both cellular respiration and fermentation.

GlycolysisGlycolysis: From Glucose to Pyruvate The energy-investing reactions of glycolysis use two ATPs per glucose molecule and eventually yield two glyceraldehyde 3- phosphate molecules. In the energy- harvesting reactions, two NADH molecules are produced, and four ATP molecules are generated by substrate-level phosphorylation. Two pyruvates are produced for each glucose molecule. Review

Glycolysis - Energy In

GlycolysisGlycolysis - Energy Out

So … How much in? 2 ATP, a glucose and 2 NAD + How much out? 4ATP – 2ATP = 2ATP 2NADH 2 Pyruvate molecules (and a couple of water to boot!)

GLYCOLYSISGLYCOLYSIS PRODUCTS 2 ATPs are used in steps 1 & 3 to prepare glucose for splitting. F 1,6-BP splits into DHAP and G3P. DHAP converts to G3P. 2 NADH are formed in step 6. 2 ATP are formed by substrate-level phosphorylation in both steps 7 and pyruvates are produced in step 10.

Pyruvate Oxidation When O 2 is available (aerobic respiration)

A multi-enzyme complex catalyzes the following three changes: 1.the carboxyl group of pyruvate is removed as a CO 2 molecule. This is a decarboxylation reaction catalyzed by the enzyme pyruvate decarboxylase.pyruvate decarboxylase 2.The 2-C fragment is oxidized to form an acetate ion. Electrons from this reaction are picked up by NAD + which is reduced to form NADH + H + 3.The acetyl group of the acetate ion is transferred to coenzyme A, forming acetyl CoA

Pyruvate Oxidation Occurs in matrix of mitochondria. 2 pyruvate + 2NAD CoA  2 acetyl-CoA + 2NADH + 2H + + 2CO 2

2 pyruvate + 2 NAD+ + 2 CoA > 2 acetyl- CoA + 2 NADH + 2 H CO2

Krebs Cycle 8 steps that oxidize acetyl-CoA to CO 2 and H 2 O, forming a molecule of ATP. In addition, the cycle removes electrons, which are carried by 3 NADH and 1 FADH 2 molecules to the ETC. The following is the overall chemical equation: oxaloacetate + acetyl-CoA + ADP + Pi + 3NAD+ + FAD ---> CoA + ATP + 3 NADH + 3H+ + FADH2 + 2CO2+ oxaloacetate

Production of Citrate The energy in acetyl CoA drives the reaction of acetate with oxaloacetate to produce citrate. The citric acid cycle is a series of reactions in which citrate is oxidized and oxaloacetate regenerated. It produces two CO 2, one FADH 2, three NADH, and one ATP for each acetyl CoA.

Occurs twice for each molecule of glucose, 1 for each acetyl-CoA. Animation: How the Krebs Cycle Works (Quiz 1)

What’s going on?? 1.In step 1, acetyl-CoA combines with oxaloacetate to form citrate. 2.In step 2, citrate is rearranged to isocitrate. 3.NAD + is reduced to NADH in steps 3, 4 and 8. 4.FAD is reduced to FADH 2 in step 6. 5.ATP if formed in step 5 by substrate-level phosphorylation. The phosphate group from succinyl-CoA is transferred to GDP, forming GTP, which then forms ATP. 6.In step 8, oxaloacetate is formed from malate, which is used as a reactant in step 1. 7.CO 2 is released in steps 3 and 4.

Complete Overview respiration

The Electron Transport Chain NADH and FADH 2 molecules derived from glycolysis and the Kreb ’ s Cycle each contain electrons they gained from their formation NADH (or FADH 2 ) molecules carry these electrons to the inner membrane of the mitochondrion and electron transfer begins*NAD+ and FAD = electron carriers – takes H ’ s (and their e-) to electron transport chain!

REDOX Ù These electrons are passed through a series of electron carriers, one step at a time. As the electrons are passed along, one substance is oxidized, the other is reduced. (REDOX rxns) *What is the “ driving force ” ? Ù At each step some energy is released. Ù This energy is used in certain places in the chain to pump protons (H + ) from the matrix out into the inner membrane space.

ETC

Electron Transport Chain (ETC) A series of electron acceptors (proteins) are embedded in the cristae. These proteins are arranged in order of increasing electronegativity. The weakest attractor of electrons (NADH dehydrogenase) is at the start of the chain and the strongest (cytochrome oxidase) is at the end.

These proteins pass electrons from NADH and FADH 2 to one another through a series of redox reactions. ETC protein complexes are alternately reduced and oxidized as they accept and donate electrons.

Energy Pumping As the electrons pass from one molecule to the next, it occupies a more stable position. The free energy released is used to pump protons (H+) to the intermembrane space. 3 for every NADH and 2 for every FADH 2. This creates an electrochemical gradient, creating potential difference (voltage) similar to a battery.

ETC

NADH and FADH 2 transfer the electrons they got from glucose to the ETC.  The electrons move through a series of redox reactions.  Energy is released and used to pump protons (H+ ions) from the matrix into the intermembrane space. At the end of the chain, the electrons are so stable that only a oxygen is strong enough to oxidize the last protein complex. Oxygen strips 2 electrons from the final protein complex and forms water with 2 protons from the matrix.

Electron Transport Chain

ATP Synthase Protons enter the matrix through proton channels associated with ATP synthase (ATPase). For every H + that passes through, enough free energy is released to create 1 ATP from the phosphorylation of ADP. Conditions must be aerobic because oxygen acts as the final electron and H + acceptor (water is formed as a byproduct).

Oxidative phosphorylation

Chemiosmosis

Synthesis of ATP Protons begin to accumulate in the intermembrane space which creates an electrochemical gradient that stores free energy known as a proton-motive force (PMF). This gradient has 2 components: 1.an electrical component caused by a higher positive charge 2.a chemical component caused by a higher concentration of protons

These protons are unable to diffuse through the phospholipid bilayer and are forced to pass through ATP synthase (ATPase). When protons move through ATPase, the free energy drives the synthesis of ATP from ADP and Pi found in the matrix (oxidative phosphorylation).ATPase ATP is then transported out into the cytoplasm by facilitated diffusion and can be used to drive endergonic processes such as movement and active transport.

The Respiratory Chain: Electrons, Proton Pumping, and ATP The chemiosmotic mechanism couples proton transport to oxidative phosphorylation. As the electrons move along the respiratory chain, they lose energy, captured by proton pumps that actively transport H + out of the mitochondrial matrix, establishing a gradient of proton concentration and electric charge—the proton-motive force.

ATPase - Chemiosmosis The proton-motive force causes protons to diffuse back into the mitochondrial interior through the membrane channel protein ATP synthase, which couples that diffusion to the production of ATP. VCAC: Cellular Processes: ATP Synthase: The Movie

Review 1.Glycolysis: occurs in the cytoplasm: anaerobic 2.Pyruvate enters the mitochondrion, converts to Acetyl-CoA 3.Kreb ’ s Cycle: Acetyl-CoA broken down into CO 2 … drives production of NADH, FADH 2 and ATP 4.Electron Transport Chain: NADH powers the electron transport system that chemiosmotically produces ATP

Aerobic Resp. Balance Sheet

ATP YIELD The theoretic yield of ATP per glucose molecule is about 36. However, it turns out that the actual yield is only 30 realistically. (Due to the fact that NADH produces an average of 2.5, not 3 and FAD produces an average of 1.5, not 2). Efficiency of energy conversion for aerobic respiration is much higher than the 2.2% of glycolysis. It is 32% efficient for aerobic. This allows multicellular organisms to exist. (compare to a car engine that is about 25% efficient!)

Metabolic Rate and BMR Metabolic rate: the amount of energy consumed by an organism in a given amount of time (increases when work is done). BMR (Basal Metabolic Rate): the minimum amount of energy needed to keep an organism alive. This is dependent on age, growth, development. This is a baseline for an organism's metabolic rate. determining Calorie needs -

Controlling Aerobic Respiration Regulated by feedback inhibition and product activation loops. Phosphofructokinase (PFK) is an allosteric enzyme that catalyzes the third reaction in glycolysis and is inhibited by ATP and stimulated by ADP. If citrate accumulates, some will enter the cytoplasm and inhibit PFK to slow down glycolysis. As citrate is used up, its concentration will decrease and the rate of glycolysis will increase.

Feedback inhibition A high concentration of NADH indicates that the ETCs are full of electrons and ATP production is high. NADH allosterically inhibits an enzyme that reduces the amount of acetyl-CoA that is shuttled to the Krebs cycle, reducing the amount of NADH produced.

Controlling Aerobic Respiration Speed up: ·  n increase in ADP ·a  decrease of citrate ·Both act on phosphofructokinase Slow down: ·  n increase in ATP (acts on phosphofructokinase) ·an increase in NADH (acts on pyruvate decarboxylase)TP