SYSTEMATICS OF THE MESOCHORINAE (INSECTA: HYMENOPTERA: ICHNEUMONIDAE) Lee, Jong-Wook and Kyong-In Suh Department of Biology, Yeungnam University Kyong-San.

Slides:



Advertisements
Similar presentations
Phylogeny of the order Corallimorpharia (Cnidaria: Hexacorallia)
Advertisements

Classification of Organisms
LG 4 Outline Evolutionary Relationships and Classification
Phylogenetics and Host Relationships of Plantbugs Inhabiting Native Cypress Pine Celia Symonds The University of Sydney and the Australian Museum Honours.
 DNA barcoding using the cytochrome c oxidase I (COI) gene will allow species identification within the genus Agrilus. This will allow the positive identification.
Phylogeny Systematics Cladistics
THE EVOLUTIONARY HISTORY OF BIODIVERSITY
1 General Phylogenetics Points that will be covered in this presentation Tree TerminologyTree Terminology General Points About Phylogenetic TreesGeneral.
Phylogenetic Trees Systematics, the scientific study of the diversity of organisms, reveals the evolutionary relationships between organisms. Taxonomy,
Phylogenetic Trees Understand the history and diversity of life. Systematics. –Study of biological diversity in evolutionary context. –Phylogeny is evolutionary.
Plant Molecular Systematics (Phylogenetics). Systematics classifies species based on similarity of traits and possible mechanisms of evolution, a change.
Reconstructing and Using Phylogenies
Classification The grouping of organisms based on characteristics
Chapter 26 – Phylogeny & the Tree of Life
© Wiley Publishing All Rights Reserved. Phylogeny.
BIOE 109 Summer 2009 Lecture 4- Part II Phylogenetic Inference.
Finding the nearest relatives of Nasonia (Hymenoptera: Pteromalidae) Roger Burks University of California, Riverside Department of Entomology.
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes Fulgerator Paul Hebert, Erin Penton, John Burns,
CLASSIFICATION & NOMENCLATURE of VIRUSES A large number of morphologically and physico ‑ chemically distinct types of viruses that infect virtually all.
Genetic Coalescence in North American White Pines Kathleen Farrell Dr. Aaron Liston, Dr. Richard Cronn, John Syring P. strobus.
CHAPTER 25 TRACING PHYLOGENY. I. PHYLOGENY AND SYSTEMATICS A.TAXONOMY EMPLOYS A HIERARCHICAL SYSTEM OF CLASSIFICATION  SYSTEMATICS, THE STUDY OF BIOLOGICAL.
JYC: CSM17 BioinformaticsCSM17 Week 4: Evolutionary systems Evolution and phylogeny Historical systems Modern methods Tools and software.
Chapter 2 Opener How do we classify organisms?. Figure 2.1 Tracing the path of evolution to Homo sapiens from the universal ancestor of all life.
JYC: CSM17 BioinformaticsCSM17 Week 4: Evolutionary systems Evolution and phylogeny Historical systems Modern methods Tools and software.
Topic : Phylogenetic Reconstruction I. Systematics = Science of biological diversity. Systematics uses taxonomy to reflect phylogeny (evolutionary history).
Phylogeny & The Tree of Life. Phylogeny  The evolutionary history of a species or group of species.
DIFFERENTIATION AND SYSTEMATICS IN THE LONG-TAILED GROUND SQUIRREL, SPERMOPHILUS UNDULATUS (SCIURIDAE, RODENTIA) Marina V. Tsvirka, Vladimir P. Korablev,
DNA in classifying species. Traditional classification Classification of organisms into closely related species, then more distant genuses, phyla and.
Phylogenetic analyses Kirsi Kostamo. The aim: To construct a visual representation (a tree) to describe the assumed evolution occurring between and among.
Phylogeny Estimation: Traditional and Bayesian Approaches Molecular Evolution, 2003
Molecular phylogenetics
BIOLOGICAL CLASSIFICATION. Taxonomy  Biological classification, or scientific classification in biology, is a method by which biologists group and categorize.
P HYLOGENETIC T REE. OVERVIEW Phylogenetic Tree Phylogeny Applications Types of phylogenetic tree Terminology Data used to build a tree Building phylogenetic.
DNA barcoding: bane or boon (or both) for taxonomy? Donal A. Hickey, Concordia University, Montreal. Collaborators: Mehrdad Hajibabaei and Gregory Singer.
FISH SPECIES IDENTIFICATION AND BIODIVERSIFICATION IN ENUGU METROPOLIS RIVER BY DNA BACODING PRESENTED BY Chioma Nwakanma (PhD) Michael Okpara University.
Phylogenetic Trees  Importance of phylogenetic trees  What is the phylogenetic analysis  Example of cladistics  Assumptions in cladistics  Frequently.
Chapter 26 Phylogeny and the Tree of Life
Figure 1. Map depicting the supposed evolutionary history of terrestrial leeches in North America. Shaded area represents the Appalachian Range. (●) Haemopis.
Phylogeny & the Tree of Life
Ayesha M.Khan Spring Phylogenetic Basics 2 One central field in biology is to infer the relation between species. Do they possess a common ancestor?
Phylogenetic analysis of flatfish species (Teleostei, Pleuronectiformes) based on cytochrome oxidase 1 (Co-1) and cytochrome b (Cyt-b) genes Sharina S.N.,
The Big Issues in Phylogenetic Reconstruction Randy Linder Integrative Biology, University of Texas
Phylogeny and Systematics Phylogeny Evolutionary history of a species of a group of related species Information used to construct phylogenies.
Higher Human Biology Unit 1 Human Cells KEY AREA 5: Human Genomics.
Reconstructing and Using Phylogenies 16. Concept 16.1 All of Life Is Connected through Its Evolutionary History All of life is related through a common.
Molecular Evolution. Study of how genes and proteins evolve and how are organisms related based on their DNA sequence Molecular evolution therefore is.
Phylogenetic trees. 2 Phylogeny is the inference of evolutionary relationships. Traditionally, phylogeny relied on the comparison of morphological features.
Phylogeny & Systematics The study of the diversity and relationships among organisms.
Identification of Drosophila species based on 16S rRNA and CO1 gene sequences Mohammad Shamimul Alam, Khandaker Asif Ahmed, Rowshan Ara Begum, and Reza.
Introduction to Bioinformatics Resources for DNA Barcoding
Introduction Conclusion References Aim of the work
Evolutionary genomics can now be applied beyond ‘model’ organisms
Phylogenetic basis of systematics
Phylogeny & the Tree of Life
Department of Biological Sciences
Gene-sequence analysis reveals at least three species hidden in Zausodes arenicolus Erin Easton November 13, 2008.
Phylogenetic Inference
Phylogeny and the Tree of Life
Molecular Evolution.
18.2 Modern Systematics I. Traditional Systematics
Chapter 25 – Phylogeny & Systematics
Volume 5, Issue 10, Pages (October 1995)
Chapter 19 Molecular Phylogenetics
18.2 Modern Systematics I. Traditional ______________
Bioinformatics CSM17 Week 4: Evolutionary systems
Molecular data assisted morphological analyses
Phylogenetic tree of 38 Pseudomonas type strains, based on the V3-V5 region sequence of the 16S rRNA gene (V3 primer, positions 442 to 492; and V5 primer,
Phylogenetic analysis of AquK2P.
Phylogenetic tree representation of a neighbor-joining analysis of several species of piroplasms. Phylogenetic tree representation of a neighbor-joining.
Molecular phylogenetic analysis of RNA polymerase II largest-subunit protein sequences from various trichomonads, including D. fragilis. Molecular phylogenetic.
Presentation transcript:

SYSTEMATICS OF THE MESOCHORINAE (INSECTA: HYMENOPTERA: ICHNEUMONIDAE) Lee, Jong-Wook and Kyong-In Suh Department of Biology, Yeungnam University Kyong-San , Korea

Class Insecta Order Hymenoptera Family Ichneumonidae Subfamily Mesochorinae

 World: 36 subfamilies, 60,000 species  Eastern Paleartic: 22 subfamilies, 15,000 species  Korea: 16 subfamilies, 355 species  Japan: ???  Parasitoid of a living arthropod Family Ichneumonidae

 World wide distribution  10 genera, about 600 species in the world  5 genera, 70 species from Eastern paleartic  Koinobiont hyperparasitoids of ecto- or endoparasitic Ichneumonoidea or Tachinidae  Several species recorded as primary endoparasitoids of lepidoptera Subfamily Mesochorinae

 World Gravenhorst (1829) Ashmead (1903) Cameron (1907), Cushman (1927, 1934) Dasch (1971, 1974) Shaw (1993)  Eastern Palearctic Uchida (1928, 1929, 1933, 1942) Nakanish (1969) Kusigemati (1967, 1985) Chao (1976) Lee and Suh (1991, 1993, 1994, 1997, 1999) Taxonomic History

 Small to large (fore wing 2-14 mm long) Diagnosis 전자현미경 사진

 Clypeus usually not separated from supraclypeal area by groove (or groove indistinct), apical margin evenly convex and without teeth 전자현미경 사진

 Sternaulus of mesopleuron short or absent 전자현미경 사진

 Areolet of fore wing large and usually rhombic 전자현미경 사진

 Metasomal segment 1 slender, glymma large and deep, spiracle near or behind middle; Metasoma of female usually somewhat laterally compressed 전자현미경 사진

 Hypopygium of female large and triangular in profile, not or barely extending beyond metasomal apex, folded on midline 전자현미경 사진

 Ovipositor needle-like, dorsal subapical notch absent; male gonoforceps extended into long and narrow rod 전자현미경 사진

 No study about revision of subfamily Mesochorinae from Eastern Palearctic region  No intensive phylogenetic study about the generic level within subfamily Mesochorinae - Need study about new microstructural characters - Need advanced morphological and molecular phylogeny

Revises the subfamily Mesochorinae for the Eastern Palearctic region, and explores the species richness and the phylogenetic relationships of the group on a world-wide basis. Objectives:

Revision of the subfamily Mesochorinae from the Eastern Palearctic region

Materials  More than 5,000 specimens were observed in this study  Specimens (including types) were assembled - by field collection - by loaning from major insect museums and collections in the world

 70 recorded species were confirmed  8 new species were described  6 unrecorded species were included in the Eastern Palearctic region Classification and Description

5 recorded species No new species No unrecorded species Total 5 species Genus Cidaphus Foerster, 1868.

Genus Astiphromma Foerster, recorded species 4 new species 2 unrecorded species Total 22 species

Astiphromma n.sp. 1 그림 입력

Astiphromma n.sp. 2 그림 입력

Astiphromma n.sp. 3 그림 입력

Astiphromma n.sp. 4 그림 입력

Genus Mesochorus Gravenhorst, recorded species 4 new species 4 unrecorded species Total 45 species

Mesochorus n.sp. 1 그림 입력

Mesochorus n.sp. 2 그림 입력

Mesochorus n.sp. 3 그림 입력

Mesochorus n.sp. 4 그림 입력

8 recorded species No new species No unrecorded species Total 8 species Genus Stictopisthus Foerster, 1886.

Genus Plectochorus Uchida, recorded species No new species No unrecorded species Total 4 species

Total 5 genera, 84 Species are recorded from Eastern Palearctic region Subfamily Mesochorinae

Phylogeny of the Subfamily Mesochorinae Based on Morphological and Molecular data

 Phylogeny based on the Morphological data 

 Ingroup: Subfamily Mesochorinae Cidaphus alarius (G.) Astiphromma dorsale (H.) Mesochorus discitergus (S.) Stictopisthus chinensis U. Plectochorus iwatensis (U.)  Outgroup: Subfamily Metopiinae Metopius sp Materials

 21 morphological characters were used  Phylogenetic inference: - Maximum Parsimony analysis and - Bootstrap analysis (1,000 replications) Using PAUP* 4.0b1 (Swofford, 1998) Method

 Phylogenetic tree based on the Maximum Parsimony analysis of the Morphological data  Tree length = 35, CI = 0.91, RI = 0.87  bootstrap value above branch

 Phylogeny based on the Molecular data 

Mitochondrial coding genes  Cytochrome b: 424 bases were sequenced  Cytochrome Oxidase I: 430 bases were sequenced

 INGROUP Subfamily Mesochorinae Cidaphus koreensis L. Korea (from Dried specimen) Astiphromma strenuum T. USA (from EtOH) Mesochorus discitergus S. Korea (from Dried specimen) Stictopisthus sagamensis L&S Korea (from Dried specimen)  OUTGROUP Subfamily Metopiinae Metopius (M.) sp. USA (from EtOH) Materials

 DNA Extraction standard procedures for Phenol- Chroloform extraction (Sambrook et al., 1989)  Amplification - PCR: after an initial denaturation step of 30s at 94  C, 35 cycle: 60s at 90  C, 60s at  C and 60s at 72  C - Primers (Simon, C. 1994; Dowton et al. 1997) : CB-J-10933(5'-TATGTACTACCATGAGGACAAATATC) and CB-N-11367(5'- ATTACACCTCCTAATTTATTAGGAAT) for Cytochrome b, CI-J-2183(5'-CAACATTTATTTTGATTTTTTGG) and MD(5'- ATTGCAAATACTGCACCTAT) for Cytochrome Oxidase I.  Sequencing AutoDNAsequencer (Perkin-Elmer ABI Prism 377) Method

 Sequence Analyses and Phylogenetic inferences - Editing and proofroading SeqApp version 1.9 (Gilbert, 1993) - Alignment of sequences Clustal W.(Thompson et al. 1994) - Calculate statistical data MEGA 1.0(Kumar et al, 1993) MacClade 3.04(Maddison & Maddison, 1992) - Maximum Parsimony and Maximum Likelihood PAUP* 4.0b1 (Swofford, 1998) - Bootstrap analysis (1,000 replications) PAUP* 4.0b1 (Swofford, 1998)

 Phylogenetic tree of CB based on MP, ML and Bootstrap analyses  Tree length = 189, CI = , RI =  -Ln likelihood =  bootstrap value above branch

 Phylogenetic tree of CB and COI combined data based on MP, ML and Bootstrap analyses  Tree length = 315, CI = , RI =  -Ln likelihood =  bootstrap value above branch

Future directions  Revision of mesochorine wasps based on the world  Find New morphology and molecular characters  Analysis of the combined molecular and morphological data  Re-establish the phylogenetic relationships of Subfamily Mesochorinae