ARMYDA-RECAPTURE (Atorvastatin for Reduction of MYocardial Damage during Angioplasty) trial Prospective, multicenter, randomized, double blind trial investigating efficacy of atorvastatin reload in patients on chronic statin therapy undergoing PCI Principal Investigators: Giuseppe Patti, Vincenzo Pasceri, Achille Gaspardone, Giuseppe Colonna Investigators: Andrea D’Ambrosio, Marco Miglionico, Annunziata Nusca, Rosetta Melfi, Laura Gatto, Elisabetta Ricottini, Gianluca Pendenza, Antonio Montinaro Chairman: Germano Di Sciascio

5 18 P=0.025 MI (%) PlaceboAtorvastatin Pasceri V, Di Sciascio G, et al. Circulation 2004 ARMYDA-RECAPTURE Background ( statin- naïve pts ) ARMYDA trial 5 17 P=0.01 MACE (%) PlaceboAtorvastatin ARMYDA-ACS trial Patti G, Di Sciascio G, et al. J Am Coll Cardiol 2007

It is unknown whether acute pretreatment in patients on chronic statin therapy would have clinical benefits similar to those observed in statin-naïve pts. In the rat model of ischemia/reperfusion, the protective effect of atorvastatin on myocardial injury wanes with longer treatment, but this effect can be “recaptured” by a reloading given immediately before ischemia/reperfusion Supplementary dose given a few hours before ischemia/reperfusion Mensah K et al. J Am Coll Cardiol 2005;45:1287 ARMYDA-RECAPTURE: Animal Studies and rationale

ARMYDA-RECAPTURE trial: Study design 793 Patients with stable angina or NSTE-ACS undergoing coronary angiography Randomization (N=420) Atorvastatin reload: 80 mg 12 hrs before angio; further 40 mg 2 hrs before N=210 Coronary angiography Placebo 12 hrs before angio; further dose 2 hrs before N=210 Primary end point: 30-day occurrence of cardiac death, MI, TVR 1 st blood sample (before PCI) CK-MB, Troponin-I, HS-CRP 2 nd and 3 rd blood samples (8 and 24 hours after PCI) 30 days 373 patients excluded for: no chronic statin therapy (31%) - 38 emergency angiography - 82 ejection fraction <30% - 10 severe renal failure PCI atorvastatin N=177 PCI placebo N= patients excluded for indication to: - medical therapy (N=30) - bypass surgery (N=38) N=352

ARMYDA-RECAPTURE: STUDY ENDPOINTS Primary endpoint  30-day incidence of cardiac death, MI, TVR - MI definition: according to the Consensus statement of the Joint ESC/ACCF/AHA/WHF Task Force, as a post procedural increases of cardiac biomarkers (troponin or CK-MB) greater than 3 x 99 th percentile of the upper reference limit in patients with normal baseline levels, and as a subsequent elevation of more than three-fold from baseline value in patients with raised baseline levels (Normal limits: CK-MB 3.6 ng/ml; Troponin-I 0.06 ng/ml) Secondary endpoints Post-procedural increase of markers of myocardial injury above UNL (CK-MB, troponin I) Post-PCI variations from baseline of CRP levels in the 2 arms

ARMYDA-RECAPTURE trial Inclusion criteria: Patients on chronic (>30 days) statin therapy and stable angina or NSTE-ACS undergoing coronary angiography Exclusion criteria:  ST- segment elevation acute myocardial infarction  Non ST-segment elevation acute coronary syndrome with high risk features warranting emergency coronary angiography (<2 hours)  Any increase in liver enzymes (AST/ALT)  Left ventricular ejection fraction <30%  Severe renal failure with creatinine >3 mg/dl  History of liver or muscle disease

VariableAtorvastatin (N=177) Placebo (N=175) P Male sex133 (75)147 (84)0.054 Age (years)66± Diabetes mellitus62 (35) 60 (34)0.97 Systemic hypertension138 (78)148 (85)0.15 Hypercholesterolemia147 (83)147 (84)0.92 Current smokers36 (20) 50 (29)0.09 LDL-cholesterol (mg/dL)92±1593± Duration of statin therapy (months)9.1±8.89.2± Serum creatinine (mg/dL)1.01± ± Clinical pattern: Chronic stable angina95 (54)94 (54)0.92 NSTEMI82 (46)81(46)0.92 Multivessel coronary artery disease83 (47)93 (53)0.29 Type of chronic statin therapy Atorvastatin98 (55)95 (54)0.92 Simvastatin (+/- ezetimibe)62 (35)58 (33)0.79 Rosuvastatin10 (6)13 (7)0.65 Pravastatin7 (4)9 (5)0.78 Other medical therapy Aspirin176 (99)175 (100)1 Clopidogrel177 (100)175 (100)- Beta-blockers73 (41)66 (38)0.57 Ace-inhibitors or ARBs117 (66)128 (73)0.19 ARMYDA-RECATURE: Clinical Features

VariableAtorvastatin (N=177) Placebo (N=175) P Vessel treated Left main2 (1) 0.63 Left anterior descending90 (42)93 (44)0.72 Left circumflex64 (30) 52 (25)0.28 Right coronary artery56 (26)57 (27)0.91 Saphenous vein grafts2 (1)6 (3)0.27 Restenotic lesions17 (10)18 (10)0.97 Lesion type B2/C97 (55)93 (53)0.84 Multivessel intervention32 (18) 0.93 Type of intervention Balloon only13 (7)11 (6)0.86 Stent164 (93)164 (94)0.86 Bifurcations with kissing balloon4 (2) 0.73 No. of stents per patient1.4±0.81.3± Use of drug eluting stents58 (33)64 (37)0.52 Use of Glycoprotein IIb/IIIa inhibitors21 (12) 0.90 Anti-thrombin therapy during intervention Unfractionated heparin159 (90)155 (89)0.84 Bivalirudin18 (10)20 (11)0.84 ARMYDA-RECATURE: Procedural Features

ARMYDA-RECAPTURE: PRIMARY ENDPOINT (30-day MACE) P=0.045 MACE (%) PlaceboAtorvastatin

Individual and Combined Outcome Measures of the Primary Endpoint at 30 days P=0.045 ARMYDA-RECAPTURE: RESULTS % Composite Primary End Point Cardiac death MI TVR MACE Atorvastatin Placebo

Individual Components of the Primary Endpoint 30-day events in the Atorvastatin and Placebo Arms Atorvastatin (N=177) Placebo (N=175) P Death- 1 (0.5%) Myocardial infarction6 (3.4%) 16 (8.6%)0.06 Stent thrombosis Target vessel revascularization (0.5%) Total MACE6 (3.4%) 15 (9.1%)0.045 ARMYDA-RECAPTURE: RESULTS

Creatine kinase-MB (%) Troponin-I (%) ARMYDA-RECAPTURE: Secondary endpoints Proportion of patients with any post-PCI cardiac markers elevation P=0.023 P= AtorvastatinPlacebo AtorvastatinPlacebo 36 47

2.1 ± ± 9.5 P=0.12 mg/L ARMYDA-RECAPTURE: Secondary endpoints Post-PCI increase of CRP levels from baseline Atorvastatin Placebo

Atorvastatin Placebo Days after PCI MACE-free survival (%) 30 P=0.045 ARMYDA-RECAPTURE trial: Event-free survival at 30 days in the atorvastatin reload vs placebo arm

0 12 ARMYDA-RECAPTURE: Odds Ratio for 30-day MACE ACS LVEF <40% IIb/IIIa inhibitors ( ) 2.1 ( ) 3.2 ( ) Atorvastatin reload * 0.52 ( ) Multiple stents 2.4 ( ) * P=0.041

ARMYDA-RECAPTURE Conclusions   ARMYDA-RECAPTURE suggests that reloading with high dose atorvastatin is associated with improved clinical outcome in patients on chronic statin therapy undergoing PCI   Acute atorvastatin bolus 80 mg + 40 mg 12 hrs pre-PCI gives a 48% relative risk reduction of 30-day MACE at MV analysis (NNT= 17)   LDL-independent cardioprotective effects may be responsible of this phenomenon   These findings may support a strategy of routine reload with high dose atorvastatin early before intervention even in the background of chronic therapy

Name of the speaker: Germano DiSciascio I have the following potential conflicts of interest to report:  Consulting  Employment in industry  Stockholder of a healthcare company  Owner of a healthcare company  Other(s) X I do not have any potential conflict of interest ACC 2009 – Disclosure Slide