Lipid-dependent surface transport of the proton pumping ATPase: A model to study plasma membrane biogenesis in yeast Fan Mengdi Li Yandi

The Points Introduction Biogenesis and transport of the proton pumping H+-ATPase

The general introduction of the H+-ATPase in yeast A good model to study plasma membrane biogenesis

The general introduction The function of ATPase : maintenance of the electrochemical gradient across the membrane regulating intracellular PH symporters

How did the H+-ATPase transport to the plasma membrane? Begin: oligomerization and package Surface transport of the ATPase Raft association Stable integrate at the plasma membrane protein lipid

How did the H+-ATPase transport to the plasma membrane? Every step needs the help of these lipids: sterols sphingolipids

Step 1: oligomerization protein Protein-lipid complex lipid C26 Pma1 The ER In the ER, Pma1form a homo-oligomeric complex—the protein-lipid complex

Step 2: package Protein-lipid complex coat protein complex II(a kind of transport vesicle) Protein-lipid complex The ER The protein-lipid complex then packaged into a larger subclass of COPII

Step 3: modify of the Pma1 Protein-lipid complex Golgi body P- coat protein complex II Protein-lipid complex P- The Pma1 becomes phosphorylated on multiple serine and threonine residues

Step 4: Pma1 transport to the cell surface Golgi body coat protein complex II Protein-lipid complex The lipid raft C26 Pma1 is transported to the cell surface

How did the H+-ATPase transport to the plasma membrane? Every step needs the help of these lipids: sterols sphingolipids

Step 5: Stable integrate at the plasma membrane C26 H+ ATP Lipid raft association

Two mutant of Pma1 Affect the stability, targeting, and detergent solubility during its transport to the surface

The first mutant:Pmal-7 Golgi body coat protein complex II Protein-lipid complex Vacuole Pmal-7:mistargeted from the Golgi to the vacuole without reaching the cell surface.

The second mutant:Pmal-10 Pmal-10: fails to become stabilized on the membrane, but to delivered to the vacuole, then degradation. vacuole

Two mutant of Pma1 . The mutant indicate: lipid raft association may play important roles in maintaining protein stability at the plasma membrane.