The Cell Chapter 4 Microscopes A. Magnification – to increase the size of an image of an object B. Resolution – how clear the image appears…the more magnified, the more blurry the image (light bends) C. Types of Light Microscopes – shines light through an object 1. simple – one lens 2. compound – two lenses (what we have) a. magnification of 400 times

D. Types of Electron microscopes (EM) – uses a beam of electron D. Types of Electron microscopes (EM) – uses a beam of electron instead of light 1. Transmission Electron microscope (TEM) a. cells sliced into thin sections and stained with atoms of heavy metals which attach to certain cell structures b. can explore a cells ultrastructure – the internal anatomy c. magnification of 100,000 times 2. Scanning Electron microscope (SEM) a. cells or group of cells have been coated with a thin film of metal. b. can study the surfaces of cells

Pictures of Electron Microscope at Miami

Paramecium (SEM) Cat Flea (SEM) Paramecium (TEM)

D. Parts and Function of a compound light microscope 1. Base – supports microscope 2. Light – shines through the object 3. Stage – where the slide sits when viewing 4. Eyepiece lens – lens closest to eye (mag.10X) 5. Arm – connects the eyepiece to the base 6. Objective lens – lens closest to object a. 4X b. 10X c. 40X d. 100X 7. Diaphragm – adjusts the amount of light shining through the object (disk vs. iris) 8. Nosepiece – rotates the objective lenses 9. Coarse adjustment – brings object into the field of view 10. Fine adjustment – focuses object, increases resolution

Eyepiece Arm Coarse Adjustment Fine Adjustment Nosepiece Objective lens Stage Diaphragm Light Base