Laboratory of Bioenergetics main laboratory :3.2.11, 3.2.11a climatic chamber :3.2.11 animal rooms: 3.0.xx administrator: Paweł Koteja Evolutionary Physiology.

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Laboratory of Bioenergetics main laboratory :3.2.11, a climatic chamber : animal rooms: 3.0.xx administrator: Paweł Koteja Evolutionary Physiology Research Team

Respirometric system Sable TR3 Main location: oxygen analyzer - „Oxzilla”: - 2-channel %, res %; CO 2 analyzer - CA2A %, res %; continuous measurement on 1 or 2 ch. sequential on 8-16 ch. open flow-through or semi-closed system; from microorganism cultures (as Microoxymax) to large animals; a small climatic chamber; mobile

Example of an open- flow setup: measurement of the maximum exercise- induced rate of aerobic metabolism Respirometric system Sable TR3 Main location:

Example of an open- flow setup: measurement of the maximum exercise- induced rate of aerobic metabolism Respirometric system Sable TR3 Main location:

More devices for respirometric measurements O 2 analyzer Applied Electrochemistry (AMETEK) S3/AII: 2-channel, 0-100%, res %, very fast, stable, lab. use only 2 x O 2 Sable analyzer FC-10A: 1-channel: 0-100%, res %, slow, not stable, mobile, 12V CO 2 analyzer Sable CA10A: 1-channel, 0-10%, res %, fast, stable, mobile, 12V CO 2 and H 2 O analyzer LiCor LI-840A: Low cost – "two in one", mobile, parameters similar to CA10A Several pumps, mass flow controllers, channel multiplexers, air driers, etc.

Motorized treadmill for respirometric measurements mouse-sized animals; speed up to 6 km/h; computer controlled speed regulation; manual angle control waterproof

Activity detectors – passive infrared Custom made: 3M – Mouse Motion Monitor 64 sensors; expandable; elastic system of data acquisition time resolution 0.01 sec. mobile – animal rooms, climatic chambers

Activity detectors - mechanical Sable MAD- 1 ("seismographs") 8 sensors; elastic system of data acquisition time resolution 0.01 sec. mobile – animal rooms, climatic chambers

Video system for behavioral analyses Noldus Ethovision video cameras, multichannel recorders analog-digital converter ("frame grabber") video analysis system main result – path of animal movement detection of a few individuals in one arena detection in several arenas (with one individual in each); several characteristics of movement: speed, time in particular zones, "meandering"; automated trials for standard behavioral tests: open field Morris water maze multi-arm labyrinths

Automatic extraction apparatus Büchi B positions temperature regulation up to +150C extraction in neutral gas automated: phases of the process (extraction, rinsing, drying) various types of extraction programmable sequences

Automatic extraction apparatus Büchi B-811 Types of process

Hydrolysis apparatus Büchi B positions vials compatible with the extraction apparatus