URICOSURIC EFFECT OF INDIAN NONI Dr.N.Murugesh, Professor & HOD, Department of Pharmacology K.S.R.Institute of Dental Science And Research Thiruchengode, Tamil Nadu.

Painful Type of Arthritis Attacks Large And Small Joints Usually Attacks one Joint At a Time Caused by High Level of Uric Acid In Blood (Hyperuricaemia) Uric Acid Crystals Collect in Joints And Soft Tissues. GOUT

AIM Noni Is Effective In Joint Disorders Such Us : Rheumatoid Arthritis Polyarthrits Juvenile Arthritis Gout Gout is caused by Hyperuricaemia No Studies on Uricosuric Effect of Noni. No Studies on Uricosuric Effect of Noni. So, for the first time Uricouric Effect of Noni has been studied.

METHODS Uricosuric Effect Studied by by Phenol Red Elimination In rats Principle Uricosuric Agents Delay The Excretion of Phenol Red So Plasma Level of Phenol Red Increases

Procedure Noni Administered Orally in Rats Noni Administered Orally in Rats 30 Min. later Phenol Red (Phenol Sulphonaphathalein) 30 Min. later Phenol Red (Phenol Sulphonaphathalein) Injected I.V Though Tail Vein. Injected I.V Though Tail Vein. 2.5 ml./ kg. Saline Flushed by the same route. 2.5 ml./ kg. Saline Flushed by the same route. Blood Collected by Retro-orbital puncture At 30, Blood Collected by Retro-orbital puncture At 30, 60 and 180 min. 60 and 180 min. Blood Diluted with 2ml of Saline. Blood Diluted with 2ml of Saline. Centrifuged And Phenol Red Concentration was Centrifuged And Phenol Red Concentration was determined in supernatant spectro photo metrically. determined in supernatant spectro photo metrically.

SERUM URIC ACID SERUM URIC ACID CONCENTRATION DETRRMINED BY JOG METHOD AT 5,10,15 AQND 20 DAYS AFTER NONI ADMINISTRATION.SERUM URIC ACID CONCENTRATION DETRRMINED BY JOG METHOD AT 5,10,15 AQND 20 DAYS AFTER NONI ADMINISTRATION.

URIC CONCENTRATION IN URINE NONI FED ANIMALS MAINTAINED IN A METABOLIC CAGENONI FED ANIMALS MAINTAINED IN A METABOLIC CAGE URINE COLLECTED WAS ESTIMATED FOR URIC ACID ON THE 5 TH, 10 TH, 15 TH AND 20 TH DAY.URINE COLLECTED WAS ESTIMATED FOR URIC ACID ON THE 5 TH, 10 TH, 15 TH AND 20 TH DAY. ALL RESULTS WERE COMPARED WITH PROBENECID A STANDARD URICOSURIC AGENT.ALL RESULTS WERE COMPARED WITH PROBENECID A STANDARD URICOSURIC AGENT.

PHENOL RED ELIMINATION

DISCUSSION PHENOL RED CONCENTRATION INCREASED IN PLASMA. EFFECT COMPARABLE TO PROBENECID DECLINE IN SERUM URIC ACID CONCENTRATION EFFECT SIMILAR TO PROBENECID URIC ACID ELIMINATION IN URINE ALSO INCREASED EFFECT MORE THAN PROBENECID ALL THESE INDICATE URICOSURIC EFFECT OF NONI THESE PROVE BENEFICIAL EFFECT OF NONI IN GOUT

ANTI H.PYLORI EFFECT OF INDIAN NONI H.Pylori (Helicobacter Pylori) – Is the causative H.Pylori (Helicobacter Pylori) – Is the causative Agent in Peptic ulcer. Agent in Peptic ulcer. Anti H.Pylori Effect of Indian Noni Studied for Anti H.Pylori Effect of Indian Noni Studied for the first time. the first time. Study is in Progress Study is in Progress Results will be presented in the next Results will be presented in the next conference. conference.

THANKS Thanks Are Due ToThanks Are Due To Prof. Dr. P.I. PETER For Inviting & Providing This Golden Opportunity We Promise To Work Much More on Indian Noni