The Effect of Resveratrol on Fat Storage in C. elegans By Chris LoBello

Obesity

Adipocytes

PPARg

Chung and Kurtev (2004)

Fei and Liu (2004) Wood and Sinclair (2004) b/nature02789_F2.html &blobtype=pdf

Methodology RT-PCR to determine if SIRT1 is activated Quantum yield of fluorescent intensity determined using SensL photon counter (counts per nanosecond) Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol Group 4: 100 microliters of resveratrol N= N2 Wild Type Strain Culture Group 3: 50 microliters of resveratrol N= N2 Wild Type Strain Culture Group 2: 10 microliters of resveratrol N= N2 Wild Type Strain Culture Group 1: 1 microliter of resveratrol N= N2 Wild Type Strain Culture Control Group: 0 microliters of resveratrol N= N2 Wild Type Strain Culture Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test The Effect of Resveratrol on Fat Storage in C. elegans

Budget VendorOrder #QuantityDescriptionPriceTotal Price Carolina C. elegans culture kit$73.00 Sigma-AldrichN MG1100 MG Nile Red Staining$64.20 Sigma-AldrichR MG1100 MG Resveratrol$81.50 SensLPCDMini00201PCDMini High Performance Photon Counting Device with 20μm sensor

Purpose To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

Hypothesis Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

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