Mass spectroscopy – learning objectives Outline the early developments in mass spectrometry. Outline the use of mass spectrometry in the determination.

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Presentation transcript:

Mass spectroscopy – learning objectives Outline the early developments in mass spectrometry. Outline the use of mass spectrometry in the determination of relative isotopic masses and for identifying elements. Interpret mass spectra of elements in terms of isotopic abundances. Keywords: mass spectroscopy, fragmentation, m/z, isotope

History of mass spectroscopy

Uses of mass spectroscopy

How a mass spectrometer works

Mass spectra of elements

Key features of the spectrum There are 3 peaks in the spectrum, so there are 3 isotopes of magnesium The heights of the peaks give the relative abundance of the isotope in the sample The results can be used to find the relative atomic mass The spectrum tells us that: 73.4% is magnesium-24, 12.7% is magnesium-25, 13.9% is magnesium-26 RAM = (24x73.4) + (25x12.7) + (26x13.9) =

How many isotopes? What is the relative atomic mass? How many isotopes? What is the relative atomic mass?